Effect of treatment with anti-HMGB1 antibody or sham IgG on gut mucosal injury in mice with acetaminophen overdose. Hematoxylin-eosin staining of the terminal ileum was assessed 24 and 48 hours after acetaminophen administration. Animal model and treatment were the same as described in Figure 1 (n = 6 for each group at 24 h; at 48 h time point, n = 9 for the sham IgG group and n = 10 for the anti-HMGB1 group. A = control, B = sham IgG at 24 h, C = anti-HMGB1 at 24 h, D = sham IgG at 48 h, E = anti-HMGB1 at 48 h, magnification = 200× for each photo). Typical picture is shown. Arrow indicates the loss of mucosal epithelial line of the villi.