Figure 5

Administration of neostigmine reduces serum cytokine levels. 12 h after induction of acute liver failure by acetaminophen (600 mg/kg APAP i.p.), serum was collected from neostigmine-treated (80 μg/kg, 1 and 7 hours after application of APAP) and control mice. Cytokine concentrations of (A) IL-1β and (B) TNF-α were measured by enzyme-linked immunosorbent assay (mean ± SD; open bars, control mice, hatched bars, APAP + solvent, filled bars, APAP + neostigmine; n = 6 for each group; *p < 0.05, **p < 0.01 APAP-alone-treated mice vs. APAP + neostigmine-treated mice).