Figure 2

Up-regulation of miR-195 reduced DIEXF mRNA and protein expression. (A): Cells were transfected with 50 nM miR-195 mimics for 48 h. qRT-PCR was performed to evaluate the mRNA level of DIEXF (top). Protein expression levels were also detected by western-blotting (bottom). (B): Top: Sequence alignment of human miR-195 with 3′ UTR of DIEXF. Bottom: mutations in the 3′-UTR of DIEXF in order to create the mutant luciferase reporter construct. (C): Cells were co-transfected with miR-195 mimics or miR-control, renilla luciferase vector pRL-SV40 and DIEXF 3′UTR luciferase reporter for 48 h. Both firefly and Renilla luciferase activities are measured in the same sample. Firefly luciferase signals were normalized with Renilla luciferase signals. All results were presented as mean ± SE. * indicates significant difference compared with that of control cells (P < 0.05).