Figure 3

Hydrogen-enrich saline inhibited hepatic peroxidation induced by ischemia/reperfusion. Male Sprague Dawley rats were subjected a partial warm liver ischemia/reperfusion injury with intraperitoneal injection of either normal saline or hydrogen enriched saline at dose of 10 ml/kg 10 minutes before reperfusion. Liver damage was assessed 2 hours after reperfusion using immunohistochemistry staining of paraffin sections for HNE or 8-OH-G (original magnification × 400), and hepatic tissue MDA measurement. An example of microphotograph stained for HNE (brown precipitation at cellular membrane) and nucleus (blue) from (A) a sham-control, (B) 2 after reperfusion with normal saline, or (C) hydrogen enriched saline treatment. An example of microphotograph stained for 8-OH-G (brown precipitation at nucleus) and nucleus (blue) from (D) a sham-control, (E) 2 hours after reperfusion with normal saline, or (F) hydrogen enriched saline treatment. (G). Percentage of peroxidative cells was significantly lower in 10 ml/kg hydrogen enriched saline treatment in comparsion of 10 ml/kg saline treatment. (*P < 0.05) (H) MDA contents obtained from liver sections subjected to reperfusion for 2, 6, 12, and 24 hours after 60 minutes ischemia with normal saline or hydrogen enriched saline treatment. Sham-operated animals underwent laparotomy only. Mean ± SEM (n = 8), *p < 0.05.