Figure 5

Effects of ozagrel on hepatic GSH depletion induced by APAP and CYP 2E1 level. (A) Mice were treated with ozagrel (200 mg/kg), NAC (600 mg/kg) or saline 30 min after the APAP (330 mg/kg) injection. The liver tissue samples were collected 0, 2 and 4 h after the APAP injection. Significant decreases in hepatic total GSH level were observed in all groups. The GSH level of APAP + NAC group was significantly higher than that of APAP group and APAP + ozagrel group at 2 h. Each value represents the mean ± S.E.M. (n = 4–5). *p < 0.001 compared with the 0 h group. ^# p < 0.001 compared with the APAP group and APAP + ozagrel group. (B) CYP 2E1 level in mouse hepatic microsomes was evaluated using a fluorogenic probe, the Vivid® CYP2E1 Blue Substrate. Ozagrel at various concentrations (0.001–1.0 mg/mL) was incubated with the reaction mixture and the fluorescence intensity of the fluorescent metabolite was measured. Each value represents the mean ± S.E.M. (n=3). There were no significant differences between the groups.