Figure 5

Flow cytometric diagrams showing total PBMCs activation (MHC-II expression) after 48 h in co-culture with a) HCC and b) non-HCC hepatocytes. c) PBMCs exhibited significant activation at 24 h and 48 h when co-cultured with HCC hepatocytes compared to non-HCC hepatocytes. Also, the activation of PBMCs in HCC co-culture was higher compared to PBMCs monoculture. Time point zero (0 h) depicts activation of total PBMCs after isolation (0 h).