Figure 2

Global gene analysis in the glandular stomach of MNU-treated mice using oligonucleotide microarray. A: Number of genes up- or down-regulated more than two-fold in the stomach of MNU-treated mice. In Venn’s diagram, the circles indicate up- (left) or down-regulated (right) genes in the stomach of MNU-treated mice with H. pylori infection, high-salt diet or their combination. The shaded area represents the up- or down-regulated genes more than two-fold only by the combination. B: Quantitative real-time RT-PCR analysis of three selected up-regulated genes (Cd177, Reg3g, and Muc13) in the stomachs of MNU-treated mice. Expression levels of the genes in each sample were normalized by Gapdh as internal control using ΔΔCT method. Relative expression levels were represented as the X-fold change relative to Group A (fixed as 1.0). Statistical analysis was performed by the Kruskal-Wallis test for general analysis and Tukey test for multiple comparison. Bars, SE; *, P < 0.01 vs. Group A and < 0.05 vs. Group C; †, P < 0.01 vs. Group C.