Figure 2

Immunohistochemical localization of PPL in normal liver. (A) Representative images for the localization of PPL and K19 in normal liver. PPL and K19 were double-stained by red and green fluorescence, respectively. Merged images are shown on the right. Scale bars: 100 μm (upper) and 25 μm (lower). (B) PPL and ZO-1 were double-stained by red and green fluorescence, respectively. Merged images are shown on the right. Arrowheads indicate PPL expression at ZO-1-negative sites. Scale bars: 100 μm (upper) and 25 μm (lower). (C) MDR and ZO-1 were double-stained by red and green fluorescence, respectively. Merged images are shown on the right. Scale bar: 25 μm. (D) PPL and γ-catenin (γ-CTN) were double-stained by red and green fluorescence, respectively. Merged images are shown on the right. Scale bar: 25 μm. (E) PPL and K19 were double-stained by red and green fluorescence, respectively. Signals for PPL were absent in cholangiocytes and hepatocytes of Ppl ^−/− mice. Scale bar: 100 μm. PV, portal vein; BD, bile duct.