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Table 2 Average plate count measurements (±SEM), expressed in log CFU mL 1 , for the different microbial groups, SHIME compartments and periods

From: Lactobacillus acidophilus CRL 1014 improved “gut health” in the SHIME®reactor

  Basal Treatment Washout
  1stweek 2ndweek 1stweek 2ndweek 3thweek 4thweek 1stweek 2ndweek
Ascending colon
Enterococcus spp 5.20a ± 0.01 5.40a ± 0.02 6.30b ± 0.10 7.25c ± 0.04 7.20c ± 0.06 8.05d ± 0.21 7.00c ± 0.02 7.00c ±0.01
enterobacteria 7.35d ± 0.41 6.00c ± 0.30 6.20c ± 0.32 6.40c ± 0.01 6.72c ± 0.22 6.80c ± 0.32 5.68b ± 0.01 4.20a ± 0.05
Lactobacillus spp 5.17a ± 0.30 5.23a ± 0.22 7.78b ± 0.03 7.77b ± 0.01 8.28c ± 0.05 7.88bc ± 0.01 7.20b ± 0.04 5.40a ± 0.04
Bifidobacterium spp 5.80a ± 0.22 6.90b ± 0.55 7.77c ± 0.01 7.86c ± 0.05 7.88c ± 0.06 7.97c ± 0.06 7.68c ± 0.03 7.61c ± 0.04
Clostridium spp 7.68a ± 0.02 8.32b ± 0.02 8.69b ± 0.01 9.39bc ± 0.01 12.39d ± 0.01 12.30d ± 0.02 12.40d ± 0.01 12.39d ± 0.01
Total aerobes 7.14a ± 0.41 7.84a ± 0.05 7.72a ± 0.01 7.42a ± 0.02 7.55a ± 0.04 7.32a ± 0.01 7.30a ± 0.01 7.42a ± 0.05
Facultative anaerobes 7.66a ± 0.03 8.20a ± 0.02 7.80a ± 0.02 7.60a ± 0.04 7.40a ± 0.01 7.30a ± 0.03 7.46a ± 0.02 7.50a ± 0.01
Transverse colon
Enterococcus spp 4.14a ± 0.22 4.44a ± 0.02 7.19b ± 0.01 6.84b ± 0.01 7.45b ± 0.03 7.50b ± 0.05 7.00b ± 0.04 7.00b ± 0.01
enterobacteria 6.23b ± 0.46 5.15a ± 0.15 5.00a ± 0.01 6.30b ± 0.01 6.00b ± 0.01 6.00b ± 0.01 6.84b ± 0.01 6.86b ± 0.02
Lactobacillus spp 5.20a ± 0.02 5.19a ± 0.02 7.72b ± 0.02 7.35b ± 0.05 7.24b ± 0.01 7.17b ± 0.05 8.33b ± 0.05 8.40b ± 0.01
Bifidobacterium spp 5.80a ± 0.07 5.00a ± 0.01 7.18b ± 0.14 7.86b ± 0.05 7.43b ± 0.01 7.31b ± 0.14 7.79b ± 0.05 7.80b ± 0.02
Clostridium spp 6.61a ± 0.01 6.62a ± 0.01 7.27a ± 0.01 8.39b ± 0.01 8.26b ± 0.03 8.53b ± 0.23 10.39c ± 0.01 10.40c ± 0.01
Total aerobes 7.00a ± 0.02 7.20a ± 0.20 7.74a ± 0.02 7.32a ± 0.11 7.82a ± 0.02 7.86a ± 0.03 8.69b ± 0.22 8.64b ± 0.10
Facultative anaerobes 6.32a ± 0.07 6.30a ± 0.01 6.00a ± 0.41 6.20a ± 0.13 6.10a ± 0.22 6.30a ± 0.01 7.80ab ± 0.01 8.10b ± 0.11
Descending colon
Enterococcus spp 4.30a ± 0.02 4.32a ± 0.01 7.08b ± 0.01 6.66b ± 0.02 6.75b ± 0.05 6.76b ± 0.04 7.08b ± 0.02 7.06b ± 0.01
enterobacteria. 7.46b ± 0.06 6.00a ± 0.01 6.15a ± 0.15 6.30a ± 0.01 6.00a ± 0.01 6.00a ± 0.01 5.67ab ± 0.01 5.70ab ± 0.01
Lactobacillus spp 5.36a ± 0.01 5.38a ± 0.02 7.46b ± 0.02 7.23b ± 0.06 7.13b ± 0.09 6.53b ± 0.53 7.20b ± 0.01 7.27b ± 0.08
Bifidobacterium spp 6.79b ± 0.04 5.72a ± 0.12 7.15c ± 0.01 7.53c ± 0.01 6.84bc ± 0.15 7.37c ± 0.03 7.55c ± 0.01 7.47c ± 0.01
Clostridium spp 7.72a ± 0.01 7.74a ± 0.06 8.48b ± 0.01 9.39c ± 0.01 10.50d ± 0.15 10.50d ± 0.02 10.34d ±0.02 10.38d ± 0.44
Total aerobes 7.24a ± 0.07 7.53a ± 0.01 7.44a ± 0.01 7.47a ± 0.42 7.58a ± 0.09 7.86a ± 0.05 8.00a ± 0.01 8.20a ± 0.45
Facultative anaerobes 7.39b ± 0.11 6.30a ± 0.01 6.59a ± 0.01 6.39a ± 0.02 6.40a ± 0.01 6.00a ± 0.03 7.69b ± 0.01 7.63b ± 0.05
  1. Different letters indicate significantly different results (p < 0.01) in same microbial group and same compartment (ascending, transverse, and descending colon), during basal, treatment and washout period.