Figure 2

Neurogenic potential of ES-derived neurospheres. ES cells (A) and ES-NS (B) were plated out onto poly-ornithine/laminin-coated plates and allowed to differentiate by culturing them in NB27 medium without growth factors for 7 days. More βIII-tubulin-positive cells (in red) are visible in ES-NS cultures as compared to ES cells differentiated under the same conditions. Nuclei are counterstained with DAPI (blue). Calibration bars = 25 μm. (C) Western blot analysis for βIII-tubulin expression in differentiated ES-NS compared to differentiated ES cells. n = 3, **P < 0.01 One Sample T test. (D) Western blot analysis for βIII-tubulin expression in differentiated ES-NS and CNS-NS. n = 3 for ES-NS and n = 4 for CNS-NSC, P = 0.83, Student’s T-test.