IR and IRS-1 mRNA levels were unchanged with HCV E2 protein expression. Huh7 cells were transfected with FLAG-CMV2 or FLAG-E2 for 24 hours. After the confirmation of successful E2 expression (A), cells were subjected to semi-quantitative PCR (B) and RT-PCR (C &D) to show IR and IRS-1mRNA expression levels were not changed by E2 expression. The error bars represented standard deviation from triplicate experiments. NS means not significant.