The inflammation in the DSS-induced model of acute colitis. (A) BW, shown as percentage of weight change, was assessed daily and represented from day 0 to day 21. Compared to the control group, the model group displayed a more weight loss of BW. (B) DAI, comprised of weight loss, stool consistency and OB, was shown. (C) Representative photographs of colon at the end of experiment were shown for control group (a), model group (b), and 1,25(OH)2D3-treated group (c). (D) Macroscopic inflammation was assessed and shown. (E) The length of the colon was quantitated and shown. (F) MPO enzymatic activity was measured and shown. (G) Representative H&E stained histology from control group (a), model group (b) and 1,25(OH)2D3-treated group (c) (H&E staining; original magnifications, ×200). (H) Colon histological scores in control, model and 1,25(OH)2D3-treated group were shown. (I and J) The total number of mononuclear cells from MLNs and LP was more in the model group compared with that in the control group. Data were expressed as mean ± SD. a
P < 0.01 vs model group; b
P < 0.01 vs control group.