Figure 2

Dectin-1 involvement in DSS induced colitis. Mice were given 1.5% DSS in their drinking water for 7 days. A) Bodyweights were measured as indication of disease severity in this model, weights are shown as percentage of weight on day seven compared to day one. B) Colons were weighed as an indication of inflammation, shown as weight per 5 cm colon. F) Longitudinally divided colons were rolled, fixed and embedded in paraffin for routine histology. Colons were evaluated by an experienced pathologist and graded from 0 to 4 points as indicated in table 1. The total inflammation score was determined by the average score of the following criteria: area involved, the number of follicle aggregates, oedema, fibrosis, hyperplasia, erosion/ulceration, crypt loss and infiltration of granulocytes and mononuclear cells. C) Representative picture of healthy colon. D) Representative picture of WT colon after 7 days of DSS induced inflammation. E) Representative picture of dectin-1-/- colon after 7 days of DSS induced inflammation. In inflamed colon a large amount of cell infiltration was found in both the mucosa and submucosa (asterisk), areas of cryptloss were clear (arrow) and we also found gut associated lymphoid tissue (arrowheads). Bar, 200 um. G) TNFα protein concentrations in colon lysates H) IL-10 protein concentrations in colon lysates I) MCP-1 protein concentrations in colon lysates. The sample means are indicated with a line. A t-test was used for statistical analysis. *, p < 0.05, n = 10.