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Table 1 Basic characteristics of the included studies

From: Association between RUNX3 promoter methylation and gastric cancer: a meta-analysis

Study/Country Mean/median age (range) (years) Gender (M/F) Patients (M+/M-) Control (M+/M-) Methods Primary Aim Methylation site RUNX3 Expression
Waki et al (2003)[26]/Japan 64.3 (0.7-89) 68/25 42/51 7/86A 4/15H MSP, RT-PCR Clarify the physiological consequence of DAP-kinase and RUNX3 age-related methylation in gastric epithelia CpG islands +
Oshimo et al (2004)[14]/Japan 68.8 (38-87) 56/24 57/23 38/7H MSP, qRT-PCR Loss of RUNX3 expression by promoter hypermethylation in Gastric Carcinoma CpG islands +
Nakase et al (2005)[27]/Japan 65.4 81/32 14/8 6/16A MSP, qRT-PCR Determine whether alteration of RUNX3 gene expression could be detected in the normal-looking gastric remnant mucosa stomach after distal gastrectomy for peptic ulcer or gastric cancer promoter hypermethylation +
Homma et al (2006)[28]/USA 63 (30-85) 28/17 43/2 43/2A MSP Clarify how methylation spreads within the CpG island region NO.1 at CpG islands -
So et al (2006)[29]/Japan 64 (30-82) 17/9 11/5 6/20A MSP, microarray Use microarray-based methylation assay to assess gene methylation CpG islands -
Mitsuno et al (2007)[30]/Japan 63.2 (26-86) 29/9 18/20 Not reported MSP Determine whether DNA methylation in six cancer-related genes affects recurrence of gastric cancer in patients who received 5-fluorouracil-based adjuvant chemotherapy CpG islands -
Gargano et al (2007)[31]/Italy Not reported 65/35 22/18 2/38A MSP Investigate a possible relationship between the RUNX3 promoter methylation, nuclear microsatellite instability and mitochondrial microsatellite instability CpG islands -
Fujii et al (2008)[32]/Japan 62.8 (52-77) 8/9 11/6 Not reported MSP, ChIP, RT-PCR Enhancer of Zeste Homologue 2 (EZH2) down-regulates RUNX3 by increasing histone H3 methylation CpG islands +
Kitajima et al (2008)[33]/Japan 65.71 34/23 30/27 10/47A MSP, PCR Determine the Helicobacter pylori infection as an independent risk factor for Runx3 methylation in gastric cancer CpG islands +
Li et al (2008)[34]/China 62 (35-78) 23/17 22/18 0/40A MSP Analyze the relationships among the aberrant methylation of RUNX3 gene promoter, the RUNX3 protein expression and clinicopathological features in gastric cancer CpG islands +
Song et al (2008)[35]/Korea 64 50/29 26/53 9/70A MSP Determine the methylation of RUNX3 promoter and the association between RUNX3 methylation and the clinicopathological characteristics of patients with gastric carcinoma CpG islands -
KIM et al (2009)[36]/Republic of Korea 57.7 53/21 18/56 2/61A MSP Comparison of DNA methylation between primary and metastatic gastric carcinoma promoter -
Zou et al (2009)[37]/China 65.15 40/17 14/2 0/20H MSP Determine the methylation frequency of 5 genes, including p16, Runx3, MGMT, DAPK, and RASSF1A CpG islands +
Chen et al (2010)[38]/China 53 (20-78) 20/50 28/42 2/68A MSP, RT-PCR Hypermethylation downregulates Runx3 gene expression and its restoration suppresses gastric epithelial cell growth by inducing p27 and caspase3 in human gastric cancer CpG islands +
Hiraki et al (2010)[39]/Japan 68.6 (45-88) 30/19 28/21 14/35A q-MS, qRT-PCR Determine whether gene methylation is a novel diagnostic marker for micrometastasis to the lymph nodes (LNs) in gastric cancer Promoter hypermethylation +
Hu et al (2010)[40]/China 64.1 97/26 68/55 12/111A MSP, RT-PCR Pathobiologic implications of methylation and expression status of Runx3 and CHFR genes in gastric cancer CpG islands +
Mikata et al (2010) [41]/Japan 70 (56-85) 14/7 10/11 4/17A MS, qRT-PCR, RT-PCR BCL2L10 hypermethylation in gastric cancer and its correlation with RUNX3 CpG islands -
  1. A: Autologous control, the control tissues from the patients themselves; H: Heterogeneous control, the control tissues from other individuals. M+: The number of tissues with methylation; M-: The number of tissues with unmethylation.