Figure 2From: Down-regulation of HSP70 sensitizes gastric epithelial cells to apoptosis and growth retardation triggered by H. pylori Stable inhibition of HSP70 in AGS cell line by siRNA. (A) AGS cells were transfected with siRNA vector targeting HSP70. a. transient transfection; b. mono-clone cell; c. stable transfection. (B) The level of HSP70 mRNA was measured by real-time PCR. Left panel showed the representative images of PCR in AGS cells transfected with siRNA/HSP70 or empty vector. Quantitative results of real-time PCR were shown in the right panel. GAPDH was used as loading control. (C) The expression profile of HSP70 in transfected AGS cells was further confirmed by immunoblotting with β-actin as an internal control. (D) Suppression of HSP70 by siRNA has inhibited AGS cell growth. Data are mean ± SD, *P <0.05, **P <0.01.Back to article page