Table 2 Primers Designs for qPCR
Gene | GENEBANK | PRIMERS (5'-3') | Amplicon Size (bp) | PROBE UPL |
|---|---|---|---|---|
TLR1 | NM_003263.3 | CCTAGCAGTTATCACAAGCTCAAA | 70 | #79 |
TCTTTTCCTTGGGCCATTC | ||||
TLR2 | NM_003264.3 | CGTTCTCTCAGGTGACTGCTC | 66 | #14 |
TCTCCTTTGGATCCTGCTTG | ||||
TLR3 | NM_003265.2 | AGTTGTCATCGAATCAAATTAAAGAG | 61 | #80 |
AATCTTCCAATTGCGTGAAAA | ||||
TLR4 | NM_138554.2 | CTGCGTGAGACCAGAAAGC | 75 | #33 |
TTCAGCTCCATGCATTGATAA | ||||
TLR5 | NM_003268.4 | GACACAATCTCGGCTGACTG | 105 | #16 |
TCAGGAACATGAACATCAATCTG | ||||
TLR6 | NM_006068.2 | TGAAACAGTCTCTTTTGSGTAAATGC | 72 | #55 |
CAGAATCCATTTGGGAAAGC | ||||
TLR7 | NM_016562.3 | CCAGTGTCTAAAGAACCTGGAAA | 63 | #5 |
TCAGGGACAGTGGTCAGTTG | ||||
TLR8 | NM_138636.2 | AGCACTTCCCTCAGGAAGATT | 62 | #27 |
NM_016610.2 | AGCACCTTCAGATGAGGCATA | |||
TLR9 | NM_017442.2 | CCAGACCCTCTGGAGAAGC | 133 | #81 |
GTAGGAAGGCAGGCAAGGT | ||||
TOLLIP | NM_019009.2 | TCCCCGCTGGAATAAGGT | 95 | #86 |
CGTCCATGGAGAAGGCTCT | ||||
TNFA | NM_000594.2 | CAGCCTCTTCTCCTTCCTGA | 123 | #29 |
GCCAGAGGGCTGATTAGAGA | ||||
IL6 | NM_000600 | GCCCAGCTATGAACTCCTTCT | 86 | #45 |
GAAGGCAGCAGGCAACAC | ||||
*RPLP0 | NM_001002.3 | ACAGGGCGACCTGGAAGT | 117 | #32 |
NM_053275.3 | GGATCTGCTGCATCTGCTT | |||
*GADPH | NM_002046.3 | AGCCACATCGCTCAGACAC | 66 | #60 |
GCCCAATACGACCAAATCC | ||||
ACTB | ENST00000331789.2 | CAACCGCGAGAAGATGAC | 121 | 560 nm |
GTCCATCACGATGCCAGT |