Figure 2

The protein levels of SCP2 in hereditary gallstone group, non-hereditary gallstone group and control group. All samples were analyzed by Western blot using anti-SCP2 antibody, and GAPDH was used as the internal control. Results were shown as SCP2/GAPDH ratio in arbitrary densitometric units representing SCP2 protein relative levels. Lane ① and ② are from control group, Lane ③ and ④ from non-hereditary group, and Lane ⑤ and ⑥ from hereditary group. SCP2 protein was expressed at higher level in hereditary cholesterol gallstone patients than that in non-hereditary cholesterol gallstone patients, and the difference was statistically significant, P < 0.05. SCP2/GAPDH ratios in hereditary, and non-hereditary cholesterol gallstone patients were 1.5898 ± 0.0316, 1.3032 ± 0.0664 respectively, which were both higher than that in control group,0.7637 ± 0.0258, P < 0.05.