Immunohistochemical analysis of superficial mucosal injury after ethanol treatment. (A) Double-color immunostaining for DCAMKL1 cells and PCNA cells. Gastric sections taken at 5 minutes (a), 1 hour (b), 6 hours (c), 24 hours (d, e) and 96 hours (f) after ethanol treatment. (B) H&E-stained sections, showing the time course of gastric mucosal damage and repair after ethanol administration. (a-f) Serial sections of the respective sections in A. Scales: 100 μm.