Hepatic TNF-α and IL-10 mRNA levels in TLR-2
-/- mice. Expression of TNF-α and IL-10 mRNA was analyzed by real-time PCR and calculated relative to average values in the control group using a comparative CT method. Data are presented as mean ± SEM of 5 observations/group. Statistical comparisons were made using two-way ANOVA and Tukey multiple comparisons test. ap < 0.05 compared to strain-matched mice fed control diet (CD); bp < 0.05 compared to diet-matched wild type (WT) mice; cp < 0.05 compared to strain-matched mice fed PUFA.