Figure 4
From: Novel application of low pH-dependent fluorescent dyes to examine colitis
Application of NBD-PZ to the mucosa of colitis induced by DSS or IL-10 deficiency. (A) Stereomicroscopic and (B) fluorescence stereomicroscopic observation of the mucosa of DSS-induced colitis in PBS containing 1 μg/ml NBD-PZ after incubation at 37°C for three minutes. A magnified image of the indicated area is shown in the lower panel. Bar, 200 μm. We observed five samples and obtained similar findings. (C) HE stain of the colonic mucosa corresponding to the indicated area in (B). (D) Stereomicroscopic and (E) fluorescence stereomicroscopic observation with 1 μg/ml NBD-PZ of the mucosa of IL-10 deficiency-induced colitis. A magnified image of the indicated area is shown in the lower panel. (F) HE stain of the colonic mucosa corresponding to the indicated area in (E).