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Figure 2 | BMC Gastroenterology

Figure 2

From: Differential detection of nuclear envelope autoantibodies in primary biliary cirrhosis using routine and alternative methods

Figure 2

Validation of substrates used in IIF for the detection of ANEA. Hep2 slides, HeLa and HepG2 cells fixed with formaldehyde either 4% (upper panel) or 1% (lower panel) and purified nuclei from HepG2 cells and rat liver were incubated with various PBC sera (s54, s32, s16, s189 and s201) and secondary anti-human FITC-labeled IgG antibodies, as described in Materials and Methods.

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