A study of best positive predictors for sustained virologic response to interferon alpha plus ribavirin therapy in naive chronic hepatitis C patients
© Idrees and Riazuddin; licensee BioMed Central Ltd. 2009
Received: 02 May 2008
Accepted: 20 January 2009
Published: 20 January 2009
The aim of this study was to determine the rate of sustained virological response (SVR) and various factors associated with response rates in chronic hepatitis C infected patients treated with interferon alpha and ribavirin combination therapy.
A retrospective review of patients data collected at this Centre from 2001 to 2007 was performed. Out of 731 consecutive patients 400 patients that fulfilled the study criteria were evaluated and subsequently treated with a combination of interferon alpha 2b (3 MU subcutaneously three injections weekly) and ribavirin (800–1200 mg orally daily). Treatment were administered for either 24 weeks or 48 weeks and patients were followed for an additional 6 months thereafter. End of the treatment response (ETR), SVR and side effects were recorded.
Out of 400 patients, 394 completed the entire treatment course and six patients discontinued treatment at month 2. Over 67% responded at the end of treatment and 16% suffered relapse. Among all treated patients, 47.6% males and 56.7% females had sustained viral response with a total combined sustained viral response rate of 50.5%. Rapid response was seen in 46.5% patients. In a multivariate logistic regression analysis, slow virological responders (adjusted OR 2.6 [95% CI 1.9–3.7]), HCV genotype 1&4 (adjusted OR 2.4 [95% CI 1.7–3.5]), pre-treatment viral load > 0.2 MIU/mL (adjusted OR 2.2 [95% CI 1.8–4.2]), Panjabi ethnic group (adjusted OR 1.6 [95% CI 1.0–3.2]) and Age > 40 years (adjusted OR 1.5 [95% CI 0.9–2.4]) were independent risk factors for non response. Side effects were usual and tolerable and only 1.5% discontinued the treatment.
The best positive predictor for SVR in this country are: rapid virologic response, HCV genotype 2 & 3, age < 40 years, ethnic race Pashtoons and pre-treatment viral load < 0.2 million IU/mL.
Hepatitis C virus (HCV) is one of the most important Flaviviridae infections in humans and is the second most common cause of viral hepatitis . Currently, almost 8–10% of the Pakistani population (MI., SR. unpublished data), 2% of the United States of America (USA) population, and an estimated 170 million people worldwide are HCV carriers . Chronic HCV infection frequently results in liver cirrhosis and is associated with an elevated risk of developing hepatocellular carcinoma . Although symptoms may be mild for decades, 20% of persistently infected individuals may eventually develop serious liver disease including cirrhosis and liver cancer . The only effective treatment is based on interferon alpha (IFN-α). Treatment with either IFN-α alone or in combination with ribavirin leads to a sustained virological response (SVR) in 20% to 56% of patients with chronic hepatitis C [4, 5]. The combination of interferon and ribavirin is the preferred treatment and achieves a better response than interferon or ribavirin alone . However, nonresponse to this therapy remains common and is associated with several factors such as HCV genotype, duration of a person's HCV infection and HCV viral load in addition to host factors such as sex, HLA type and cytokine polymorphisms [7, 8]. Patient age, grade of liver inflammation and ethnicity have also been shown to influence response to therapy [9, 10]. The strongest predictors for a SVR to treatment is the HCV genotype, with HCV genotype 1 (HCV-1) being the least sensitive to IFN-α based therapy [11, 12].
Several studies are available on the response rates to combinatorial IFN-α/ribavirin treatment of hepatitis C in Pakistan [13, 14], however, these do not describe positive and negative predictors for the SVR rates. The aim of this study was to determine the efficacy and safety of a 24 weeks or 48 weeks treatment with IFN-α plus ribavirin in patients with chronic hepatitis C genotypes none-1 and 1 respectively and to identify factors that impaired response to antiviral therapy. We focused our study on naïve patients that had not previously received antiviral treatment and who presented with HCV genotypes non-1 and 1.
A retrospective analysis was performed on data collected from 731 patients with chronic HCV infection screened between 2001 and 2007 at multiple clinics throughout Pakistan. Of the 731 consecutive screened patients, 400 patients (280 male, 120 female, mean age 38 years [range, 16–70 ± SD] years) were chronically infected with various HCV subtypes and had not received antiviral treatments previously. These 400 patients fulfilled the study criteria and were enrolled for antiviral therapy. A total of 331 patients were excluded as either they were unwilling to participate (n = 119) or failed to meet inclusion criteria for the study (n = 212). Patients were enrolled from provinces of Punjab (Panjabi; 299), North West Frontier Province (Pashtoons; 78), Sindh (Sindhi; 11) and Balochistan (Balochi; 12). Probable transmission risk factors were previous major/minor surgery (20%), transfusion of blood or blood products (2%), dental surgery (10%), or sporadic (60%). The estimated duration of infection varied from 6 months to 20 years.
Diagnosis, inclusion and exclusion criteria
The diagnosis of chronic hepatitis C was based on elevated serum transaminase levels for at least 6 months and the consistent detection of serum HCV RNA. Anti-HCV antibodies (3rd generation ELISA) were present in each patient. Prior to the start of the treatment, patients complete blood count (CBC) were measured and patients were required to have haemoglobin levels above 13 g/dL in males and 12 g/dL in females and platelet counts above 80,000. Patients were also required to have persistently elevated alanine aminotransferase (ALT), white blood cell count greater than 3.0/mm3, normal serum bilirubin, albumin, creatinine and thyroxin levels. Furthermore the patients were required to be negative for hepatitis B surface antigen, and not suffering from decompensate liver disease, autoimmune disorders, or a history of depression and/or cardiac diseases. Patients that were under 18 years or above 70 years of age and pregnant females were also excluded from the studies.
Measurement of HCV RNA and HCV genotyping
Quantitative detection of HCV RNA in patient sera during pre-treatment, treatment and post-treatment were performed using real-time PCR (SmartCycler II, Cepheid, USA) with an internal RNA standard derived from the 5' UTR. Genotyping of HCV was performed in pre-treatment sera as described previously . All measures suggested by Kwok and Higuchi  to prevent sample contamination were strictly adhered to.
All patients with HCV 2a-b, 3a-d, 5a, 6a or with mixed infection of these genotypes received 3 subcutaneous injections per week of 3 MU recombinant IFN-α plus ribavirin (10 mg/day/kg body weight) for a total of 24 weeks. All patients with HCV genotypes 1a-c, 4 or those with mixed infections of these genotypes received 3 subcutaneous injection per week of 3 MU recombinant IFN-α plus ribavirin administered orally (1,000 mg/day for patients ≤ 75 kg body weight or 1,200 mg/day for patients > 75 kg body weight) for 48 weeks. Informed consent (printed in local language) was obtained from each patient and the Ethics Committee of the Centre approved this study.
All patients that fulfilled the study criteria were monitored for HCV RNA and ALT during and after treatment. Efficacy of treatment was assessed with normalization of ALT and absence of serum HCV RNA measured at week 4, 8, 12 and 24 and being undetectable at the end of the treatment at 24 or 48 weeks which constituted end of treatment response and at the end of follow up at 12 or 18 months which constituted the sustained viral response for patients with genotypes non-1 and 1 respectively. Adverse effects were monitored during each follow up visit. The study was initiated in May 2001 and all the follow-ups were completed by June 2007. A number of study end-points were defined, based upon the copy number of HCV RNA in patient sera. End of treatment response (ETR) was defined as undetectable HCV RNA (< 100 copies/mL) at the end of treatment. Sustained virologic response (SVR) was undetectable HCV RNA 6 months after the end of treatment and non responders were those who were with detectable HCV RNA at the end of treatment. Patient relapse was defined as those whose HCV RNA was undetectable at the end of treatment but reappeared within 6 months of cessation of treatment. Rapid virologic response (RVR) was defined as undetectable HCV RNA following an initial 4 weeks of treatment.
Statistical analysis of the generated data
Clinical and biochemical characteristics of patients are expressed as a mean or median, with a standard deviation as appropriate. Summary statistics were calculated using SPSS (v10.0 SPSS Inc.). The results for all variables were given in the form of rates (%.). Chi-square and Fishers Exact test was used to measure association amongst categorical variables. P-values less than 0.05 were considered significant. Multivariate logistic regression analysis was performed to identify factors that were associated with non response to therapy. Variables associated with relapse in univariate analysis (P < 0.1) were entered into the model. Potential confounders were tested before selecting the final model by entering non-significant variables into the selected multivariate model and determined their effect on the odds ratios of the independent variables.
Study enrolments and disposition of patients
Demographic characteristics of treated patients (N = 400).
(N = 70)
(N = 33)
(N = 260)
(N = 6) *
(N = 31)†
Mean age (Y)
50 ± 9.5
48 ± 9.0
45 ± 10.2
49 ± 9.8
46 ± 8.4
Ethnic group-No. (%)
Pre-treatment HCV RNA level
< 200,000 IU/mL
> 200,000 IU/mL
Out of 400 patients, 268 patients (67%) showed end of treatment response (ETR) and 202 (50.5%) patients achieved a sustained virological response (SVR). Sixty six (16.5%) patients were HCV-RNA negative at the end of treatment but relapsed thereafter. One hundred and thirty two (33%) patients were virologic nonresponders showing detectable HCV RNA at the end of treatment. Rapid virological response was seen in 186 (46.5%) patients. Out of sixteen patients who had cirrhosis on the liver biopsy, 10 were males and 6 were females. Half of these patients had end of treatment response but all were relapsed giving no sustained viral response in these patients.
Virologic response in different ethnic groups
An ethnic difference was seen in both ETR and SVR rates. In ethnic groups Pashtoon, Panjabi, Sindhi and Balochi the rates of SVR were 69.2%, 45.5%, 45.5% and 50% respectively. The rates of SVR are significantly higher in ethnic group Pashtoon compared with Panjabi, Sindhi and Balochi patients (P = 0.026).
Predictors of sustained virologic response
Rates of NR*, ETR† and SVR‡ in patients infected by different HCV genotypes to interferon plus ribavirin therapy (N = 400).
1 (a, b, c)
2 (a, b)
3 (a, b, c, d)
Others (4, 5a, 6a)
Rates of NR*, ETR† and SVR‡ in HCV infected patients of different Age groups to interferon plus ribavirin therapy (N = 400).
≤ 20 Years
> 60 Years
Variables associated with rates of SVR‡ in chronic HCV patients treated with interferon plus ribavirin standard therapy (N = 400).
Total No. of treated patients
(n = 400)
(n = 202)
SVR rate, %
Pre-treatment viral load
< 0.2 MIU/mL
> 0.2 MIU/mL
Rapid virological response
2, 3 or mixed with 2&3
1, 4, 5, 6 or mixed
Multivariate logistic regression analysis of variables associated with decrease sustained virological response rates to alpha interferon plus ribavirin treatment in chronic HCV patients (N = 400).
SVR‡/Total No. of treated patients
Pre-treatment viral load
> 0.2 MIU/mL
< 0.2 MIU/mL
Rapid virological response
1, 4, 5, 6 or mixed with these genotypes
2, 3 or mixed with these genotypes
CI cannot be
Side effects observed during anti-viral therapy in HCV infected patients (N = 400)
Side effect observed
Influenza like symptoms
Redness at injection site
Our study of patients infected with HCV shows that the overall end of treatment response rates to interferon plus ribavirin therapy is very high (67%) in this country. In a considerable proportion of patients (16%) however, virologic relapse occurred after the completion of therapy in the follow up period. This relapse rate is very high as compared to that reported by Khokhar and Co-workers  in Pakistani patients (only 4% relapse rate) but is very low compared to that reported by Sarrazin et al.,  that was 43%. Similarly, John et al.,  have reported a response rate of 53% to combination therapy for 6 months with sustained virological response of 31%. The low response rate reported by Sarrazin et al., and John et al., may be due to the fact that genotype 1b was predominant in their study which is associated with severe clinical course of the disease and a decreased response to treatment. Conversely, in Pakistan the predominant genotype is 3a, which has a slower progression and a better response to treatment .
A number of viral and host factors have been identified during the course of the present study that may help to predict response rates of current antiviral therapy. Our data suggest that the patients slow/rapid virologic response rate, their pre-treatment HCV viral load, age, stage of fibrosis, gender, ethnicity and HCV genotypes are factors that may play an important role in determining response rates to interferon plus ribavirin treatment. The most important of these factors appears to be the achievement of undetectable level of HCV-RNA in serum by week 4, the HCV genotype and pre-treatment viral load. This data suggest that patients with a rapid virologic response (RVR) had a significantly higher sustained virologic response (SVR) rate (P < 0.05); in more than 85% of cases RVR occurred with SVR. These findings support a recent study that suggests high rates of SVR in patients shown RVR within 16 weeks and 24 weeks of therapy . As expected, it was also observed in the present study that rate of SVR differed among patients with different HCV genotypes, with higher SVR observed with HCV genotypes 2 and 3. This finding in the Pakistani population confirms previously reported work that shows a better response to IFN-alpha treatment in patients with HCV genotypes 2 and 3 [8, 22, 23] from other parts of the World. An interesting finding of the present study is that the SVR rate is significantly higher in patients with a pre-treatment viral load of less than 200,000 IU per mL than patients with pre-treatment viral load of greater than 200,000 IU/mL. Several other recent studies have shown an inverse relationship between sustained virologic response rates and pre-treatment viral load [21, 24].
Perhaps the most interesting and important finding of the present study is the observation that response rates are significantly higher (P = 0.026) in ethnic Pashtoon as compared to Panjabi. Race has also been shown to influence response to therapy by other researchers [9, 10]. This suggests that Panjabi race is an independent risk factor for the decreased rate of response to treatment of HCV infection. These data need to be conformed by further studies with larger numbers of Pashtoon patients. The baseline characteristics of Pashtoon and Panjabi patients were similar however we were unable to assess differences in body mass index and incidence of diabetes mellitus that may play a role in interferon non-responsiveness. Similar rates of SVR have been reported in Indian Panjabi 53%  compared to 45.5% seen in the present study. The 7.5% greater response rate seen in Indian Panjabi race may be due to the administration of pegylated interferon and ribavirin for treatment. Our study further shows that younger patients have higher response rates to antiviral therapy than older patients (P < 0.001) in agreement with previous studies [21–23]. Another important finding of the present study is that patients with cirrhosis had 0% SVR rates even though 85% patients with cirrhosis enrolled in this study had ETR. This observed ETR is much better than previously reported response but unfortunately all of our patients with cirrhosis were relapsed soon after treatment was discontinued.
All patients enrolled in this study tolerated the treatment well and the side effects were in line with that previously reported . Six of our patients did develop psychosis and had to discontinue treatment after 2 months of treatment, relapsing soon thereafter. Though side effects of the combination regimen are additive not synergistic, they generally do not limit the safe use of this therapy. Appropriate recognition and management of side effects will both improve therapeutic response and avoid unnecessary morbidity and mortality .
In conclusion our study shows that twenty-four weeks combination treatment with interferon-α2b and ribavirin in patients with genotypes 2 and 3 may be as useful as PEG-interferon plus ribavirin for sustained virological response. Patients with RVR, low pre-treatment viral load, HCV genotypes 2 & 3, age < 40 years and ethnic group Pashtoon appear to have the highest probability of ETR and SVR.
We thank all the gastroenterologists, clinicians and subjects for their cooperation in this study. We thank Dr. Saleem Kamili, Division of Viral Hepatitis, CDC and Dr. Zubair M. Ahmad, of NIH for critically reading the manuscript. This project was partially supported by the Ministry of Science & Technology, Government of Pakistan.
- Leiveven J: Pegasys/RBV Improves Fibrosis in Responders, relapsers & Nonresponders with Advanced Fibrosis. 55th Annual Meeting of the American Association for the Study of Liver Disease:. 2004, Boston, MA, USA, October 29–November 2Google Scholar
- Alter MJ, Kruszon-Moran D, Nainan OV, McQuillan GM, Gao F, Moyer LA, Kaslow RA, Margolis HS: The prevalence of hepatitis C virus infection in the United States, 1988 through 1994. N Engl J Med. 1999, 341: 556-562. 10.1056/NEJM199908193410802.View ArticlePubMedGoogle Scholar
- Neumann AU, Lam NP, Dahari H, Gretch DR, Wiley TE, Layden TJ, Perelson AS: Hepatitis C viral dynamics in vivo and the antiviral efficacy of interferon-alpha therapy. Science. 1998, 282: 103-107. 10.1126/science.282.5386.103.View ArticlePubMedGoogle Scholar
- McHutchison JG, Gordon SC, Schiff ER, Shiffman ML, Lee WM, Rustgi VK, Goodman ZD: Interferon alfa-2b alone or in combination with ribavirin as initial treatment for chronic hepatitis C. Hepatitis Interventional Therapy Group. N Engl J Med. 1998, 339: 1485-1492. 10.1056/NEJM199811193392101.View ArticlePubMedGoogle Scholar
- Poynard T, Marcellin P, Lee SS, Niederau C, Minuk GS, Ideo G, Bain V: Randomised trial of interferon alpha2b plus ribavirin for 48 weeks or for 24 weeks versus interferon alpha2b plus placebo for 48 weeks for treatment of chronic infection with hepatitis C virus. International Hepatitis Interventional Therapy Group (IHIT). Lancet. 1998, 352: 1426-1432. 10.1016/S0140-6736(98)07124-4.View ArticlePubMedGoogle Scholar
- Reichard O, Norkrans G, Fryden A, Brachonier JH, Sonnerborg A, Weiland O: Randomized double blind, placebo controlled trial of interferon alpha 2b with and with out ribavirin for chronic hepatitis C. Lancet. 1998, 351: 83-7. 10.1016/S0140-6736(97)06088-1.View ArticlePubMedGoogle Scholar
- Sim KH, Wojccik JP, Margulies M, Wade J, Heathcote J: Human leukocyte antigen class II alleles may affect response of chronic hepatitis C to interferon therapy. J Viral Hepat. 1998, 5: 249-253. 10.1046/j.1365-2893.1998.00111.x.View ArticlePubMedGoogle Scholar
- Davis GL, Lau JY: Factorespredictive of beneficial response to therapy of hepatitis C. Hepatology. 1997, 26 (3 Suppl 1): 122S-127S. 10.1002/hep.510260721.View ArticlePubMedGoogle Scholar
- Camps J, Crisostomo S, Garcia-Granero M, Riezu-Boj JI, Civeira MP, Prieto J: Prediction of the response of chronic hepatitis C to interferon alfa: a statistical analysis of pre-treatment variable. Gut. 1993, 34: 1714-1717. 10.1136/gut.34.12.1714.View ArticlePubMedPubMed CentralGoogle Scholar
- Hayashi J, Kishihara Y, Ueno K, Yamaji K, Kawakami Y, Furusyo N, Sawayama Y: Age-related response to interferon alfa treatment in women vs men with chronic hepatitis C virus infection. Arch Intern Med. 1998, 158: 177-181. 10.1001/archinte.158.2.177.View ArticlePubMedGoogle Scholar
- Manns MP, McHutchison JG, Gordon SC, Rustgi VK, Shiffman M, Reindollar R, Goodman ZD, Koury K, Ling M, Albrecht KJ: Peginterferon alfa-2b plus ribavirin compared with interferon alfa-2b plus ribavirin for initial treatment of chronic hepatitis C: a randomised trial. Lancet. 2001, 358: 958-965. 10.1016/S0140-6736(01)06102-5.View ArticlePubMedGoogle Scholar
- Poynard T, Marcellin P, Lee S, Niederau C, Minuk GS, Ideo G, Bain V: Randomised trial of interferon alpha2b plus ribavirin for 48 weeks or for 24 weeks versus interferon alpha2b plus placebo for 48 weeks for treatment of chronic infection with hepatitis C virus. International Hepatitis Interventional Therapy Group (IHIT). Lancet. 1998, 352: 1426-1432. 10.1016/S0140-6736(98)07124-4.View ArticlePubMedGoogle Scholar
- Wazir MS, Majid AS, Solangi GA, Zuberi BF: Role of interferon and interferon plus ribavirin in the management of chronic hepatitis C. J Coll Phys Surgeo, Pak. 2002, 12: 609-612.Google Scholar
- Shafi MS, Ahmad SI, Rehana M, et al: Daily interferon versus interferon TIW in combination with ribavirin for treatment of chronic hepatitis C. JRMC. 1999, 31: 20-2.Google Scholar
- Idrees M: Development of a New HCV Genotyping Assay and its Suitability for the Detection of Common Genotypes in Hepatitis C Patients in Pakistan. J Virol Meth. 2008, 105: 50-56. 10.1016/j.jviromet.2008.03.001.View ArticleGoogle Scholar
- Kwok S, Higuchi R: Avoiding false positives with PCR. Nature. 1989, 339: 237-238. 10.1038/339237a0.View ArticlePubMedGoogle Scholar
- Khokhar N, Asif N, Omar S: Hepatitis C virus serotype in chronic liver disease. Pak J Med Sci. 2002, 18 (2): 156-159.Google Scholar
- Sarrazin C: Mutation within E2 and NS5A protein in patients infected with Hepatitis C Virus type 3a and correlation with treatment response. Hepatology. 2001, 31 (6): 1360-70. 10.1053/jhep.2000.7987.View ArticleGoogle Scholar
- John G, Mc Hutchinisum, Straut C, Gordan , Eugene RS, Mitchell L: Interferon alpha 2b alone or in combination with ribavirin as initial treatment for chronic hepatitis C. N Engl J Med. 1998, 339: 1485-1492. 10.1056/NEJM199811193392101.View ArticleGoogle Scholar
- Idrees M: Common genotypes of hepatitis C virus present in Pakistan. Pak J Med Res. 2002, 40 (2): 46-49.Google Scholar
- Shiffman ML, Suter F, Bacon BR, Nelson D, Harley H, Solá R, Shafran SD, Barange K, Lin A, Soman A, Zeuzem S: Peginterferon Alfa-2a and Ribavirin for 16 or 24 Weeks in HCV Genotype 2 or 3. N Engl J Med. 2007, 357: 124-134. 10.1056/NEJMoa066403.View ArticlePubMedGoogle Scholar
- Lau JYN: Significance of Serum Hepatitis C Virus RNA Levels in Chronic Hepatitis C. Lancet. 1993, 341: 1501-1504. 10.1016/0140-6736(93)90635-T.View ArticlePubMedGoogle Scholar
- Kanai K: HCV Genotypes in Chronic Hepatitis C and Response to Interferon. Lancet. 1992, 339: 1543-10.1016/0140-6736(92)91311-U.View ArticlePubMedGoogle Scholar
- Hadziyannis SJ, Sette H, Morgan TR, Balan V, Diago M, Marcellin P, Ramadori G: Peginterferon-alpha2a and ribavirin combination therapy in chronic hepatitis C: a randomized study of treatment duration and ribavirin dose. Ann Intern Med. 2004, 140: 346-355.View ArticlePubMedGoogle Scholar
- Sood A, Midha V, Sood N, Bansal M: Pegylated interferon alfa 2b and oral ribavirin in patients with HCV-related cirrhosis. Indian J Gastroenterol. 2006, 25 (6): 283-285.PubMedGoogle Scholar
- Sulkowski MS, Wasserman R, Brooks L, Ball L, Gish R: Changes in Haemoglobin during Interferon alpha-2b Plus Ribavirin Combination Therapy for Chronic Hepatitis C virus Infection. J Viral Hepat. 2004, 11 (3): 243-50. 10.1111/j.1365-2893.2004.00490.x.View ArticlePubMedGoogle Scholar
- Shiffman ML: Side Effects of Medical Therapy for Chronic Hepatitis C. Ann Hepatol. 2004, 3 (1): 5-10.PubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-230X/9/5/prepub
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