Clinicopathologic characteristics of high expression of Bmi-1 in esophageal adenocarcinoma and squamous cell carcinoma
© Choy et al.; licensee BioMed Central Ltd. 2012
Received: 9 May 2012
Accepted: 8 October 2012
Published: 18 October 2012
High expression of Bmi-1, a key regulatory component of the polycomb repressive complex-1, has been associated with many solid and hematologic malignancies including esophageal squamous cell carcinoma. However, little is known about the role of Bmi-1 in esophageal adenocarcinoma. The aim of this study is to investigate the amplification and high expression of Bmi-1 and the associated clinicopathologic characteristics in esophageal adenocarcinoma and squamous cell carcinoma.
The protein expression level of Bmi-1 was detected by immunohistochemistry (IHC) from tissue microarrays (TMA) constructed at the University of Rochester from using tissues accrued between 1997 and 2005. Types of tissues included adenocarcinoma, squamous cell carcinoma and precancerous lesions. Patients’ survival data, demographics, histologic diagnoses and tumor staging data were collected. The intensity (0–3) and percentage of Bmi-1 expression on TMA slides were scored by two pathologists. Genomic DNA from 116 esophageal adenocarcinoma was analyzed for copy number aberrations using Affymetrix SNP 6.0 arrays. Fisher exact tests and Kaplan-Meier methods were used to analyze data.
By IHC, Bmi-1 was focally expressed in the basal layers of almost all esophageal squamous mucosa, which was similar to previous reports in other organs related to stem cells. High Bmi-1 expression significantly increased from squamous epithelium (7%), columnar cell metaplasia (22%), Barrett’s esophagus (22%), to low- (45%) and high-grade dysplasia (43%) and adenocarcinoma (37%). The expression level of Bmi-1 was significantly associated with esophageal adenocarcinoma differentiation. In esophageal adenocarcinoma, Bmi-1 amplification was detected by DNA microarray in a low percentage (3%). However, high Bmi-1 expression did not show an association with overall survival in both esophageal adenocarcinoma and squamous cell carcinoma.
This study demonstrates that high expression Bmi-1 is associated with esophageal adenocarcinoma and precancerous lesions, which implies that Bmi-1 plays an important role in early carcinogenesis in esophageal adenocarcinoma.
KeywordsEsophageal adenocarcinoma Bmi-1 Squamous cell carcinoma Barrett’s esophagus Dysplasia High expression Biomarker Overall survival
Esophageal carcinoma is the 8th leading cancer in incidence and 6th in mortality worldwide, but it is one of the least studied cancers [1, 2]. Squamous cell carcinoma and adenocarcinoma are the two histologic types that make up for greater than 90 percent of the diagnoses of esophageal cancers . Worldwide, the majority of esophageal cancers are squamous cell carcinoma . However, in the United States and Western countries, there has been a dramatic rise in the incidence of esophageal adenocarcinoma to equal or exceed the incidence of esophageal squamous cell carcinoma . Esophageal carcinoma carries a poor prognosis with an overall five-year survival rate of approximately 15 percent in the United States . More than 50 percent of patients have either unresectable tumors or radiographically visible metastases at the time of diagnosis . Identification of early diagnostic markers with high sensitivity and specificity will provide physicians with valuable information for diagnosis, prognosis, and possible treatment options of esophageal carcinoma. Previous studies have suggested the order of events that leads to esophageal adenocarcinoma from normal esophageal epithelium to reflux esophagitis, followed by Barrett’s esophagus, dysplasia, to esophageal adenocarcinoma . During these events, a series of genetic and epigenetic aberrations driven by inflammation and oxidative stress contributes to the carcinogenesis. However, the oncogenetic mechanisms of esophageal adenocarcinoma remain unclear.
The Bmi-1 (B cell-specific Moloney murine leukemia virus integration site 1) gene, a member of the polycomb-group proteins, was first isolated as an oncogene that cooperates with c-myc in the oncogenesis of murine lymphomas [8–10]. It functions as a transcriptional repressor through chromatin modification and plays a role in axial patterning, cell cycle regulation, hematopoiesis, and senescence [11, 12]. In addition, deregulation of polycomb-group gene expression leads to cell proliferation and tumor progression [13, 14]. Aberrant Bmi-1 expression has been associated with many solid and hematologic malignancies, including mantle cell lymphoma , Hodgkin lymphoma , B-cell non-Hodgkin lymphoma , gastric carcinoma , hepatocellular carcinoma , colorectal cancer [20, 21], breast cancer [22, 23], bladder cancer , nasopharyngeal carcinoma , oral squamous cell carcinoma  and non-small cell lung cancer . More recently, studies have reported an association between Bmi-1 expression and esophageal squamous cell carcinoma [28–30]. However, little is known about the role of Bmi-1 in esophageal adenocarcinoma.
The aims of this study are (1) to investigate the association of high Bmi-1 expression with the oncogenic progression of esophageal adenocarcinoma from squamous mucosa, columnar cell metaplasia, Barrett’s esophagus, low- and high-grade dysplasia to adenocarcinoma, and (2) to determine the relationship of high Bmi-1 expression with clinicopathologic characteristics including gender, age, differentiation, and tumor stage in both esophageal adenocarcinoma and squamous cell carcinoma.
Construction of Tissue Microarray
Tissue microarrays, containing 80 cases of squamous epithelium, 63 cases of columnar cell metaplasia, 36 cases of Barrett’s esophagus, 20 cases of low-grade dysplasia, 14 cases of high-grade dysplasia, 110 cases of esophageal adenocarcinoma, and 34 cases of esophageal squamous cell carcinoma, were constructed from representative areas of formalin-fixed specimens collected from 1997 to 2005 in the Department of Pathology and Laboratory Medicine, University of Rochester Medical Center/Strong Memorial Hospital, Rochester, NY. All research was performed under protocols approved at URMC with the title “Biomarkers of esophageal carcinoma” and RSRB case number: RSRB00028546. The 5-μm sections were cut from tissue microarrays and stained with H&E to confirm the presence of the expected tissue histology within each tissue core. Additional sections were cut for immunohistochemistry analysis.
Pathologic definition of esophageal adenocarcinoma and precancerous lesions
Columnar cell metaplasia was defined as columnar cells without goblet cell metaplasia including mucous glands or mixture of mucous and oxyntic glands. Barrett’s esophagus was defined as mucous glands with goblet cell metaplasia. Low-grade dysplasia was defined as elongated, crowded, hyperchromatic, mucin depletion and pseudo-stratified nuclei with relatively preserved crypt architecture. High-grade dysplasia was defined as marked cytologic abnormality and significant architectural complexity of the glands. Cytologic abnormalities included nuclear pleomorphism, loss of polarity, irregularity of nuclear contour, increased ratio, and increased number of atypical mitoses. Significant architectural complexities of the glands included crypt budding, branching, marked crowding or villiform contour, intraluminal papillae, bridges or a cribriform growth pattern. Esophageal adenocarcinoma was defined as the single cells, small or large irregular glands with both cytologic abnormality and architectural complexity infiltrating into submucosa or deeper layers of esophagus.
Patients for Tissue Microarrays
Distribution of patients by histologic types
Columnar cell metaplasia
Squamous cell carcinoma
Association of high Bmi-1 expression with age, gender, lymph node metastasis, stage and differentiation in esophageal adenocarcinoma
Bmi-1 Non-high expression
Lymph node metastasis
Patients for Affymetrix SNP 6.0 analysis
Frozen tumors were obtained from 116 patients undergoing esophagectomy at the University of Pittsburgh Medical Center, Pittsburgh, PA between 2002 and 2008. The patients' ages ranged from 43 to 88 and the cohort consisted of 95 males and 21 females. The final pathologic stages were stage I (28), stage II (31), stage III (49) and stage IV (7). All tumor specimens were evaluated by a pathologist and determined to be >70% tumor cell representation. Further information on this patient cohort and a comprehensive genomic analysis of these tumors were published by Dulak et al. . Microarray data on this cohort has been submitted to the Gene Expression Omnibus (GSE36460) and was made public (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE36460). A research was performed under protocols approved at both participating institutions.
Affymetrix SNP 6.0 analysis
Genomic DNA was isolated using the QiaAmp DNA Mini Kit (Qiagen, CA), and 600ng was used for labeling and array hybridization at the SUNY Upstate Medical University microarray core facility (Syracuse, NY) using kits and protocols provided by Affymetrix. Array data quality was assessed using Affymetrix Genotyping Console 3.0 and all further data analysis was performed using Nexus 5.0 Copy Number Analysis software (Biodiscovery, Inc. CA).
Tissue sections from the tissue microarray were deparaffinized, rehydrated through graded alcohols, and washed with phosphate buffered saline. Antigen retrieval for Bmi-1 was performed by heating sections in 99°C water bath for 40 minutes. After endogenous peroxidase activity was quenched and nonspecific binding was blocked, ready-to-use mouse monoclonal antibody anti-Bmi-1 (Millipore, MA) was incubated at room temperature for 30 minutes. The secondary antibody (Flex HRP) was allowed to incubate for 30 minutes. After washing, sections were incubated with Flex DAB Chromogen for 10 minutes and counterstained with Flex Hematoxylin for 5 minutes. A colon adenocarcinoma with known high Bmi-1 expression served as positive control. Negative control was performed by replacing anti-Bmi-1 antibody with normal serum. A few core samples did not survival from the immunohistochemical staining.
Scoring of Immunohistochemistry
All the descriptive statistics in this study were presented as mean. A P-value of less than 0.05 was considered statistically significant. The univariate analysis of Bmi-1 was conducted first and then followed by a multivariate analysis, including age, gender, and clinical covariate: lymph node metastasis and tumor stage. We divided esophageal adenocarcinoma, high- and low- and grade dysplasia, columnar cell metaplasia as group 1, and squamous cell carcinoma and squamous epithelium as group 2. Chi-square and Fisher exact tests were used as appropriate to compare Bmi-1 positivity rate in the two groups. To evaluate the influence of amplification and high expression of Bmi-1 in esophageal adenocarcinoma and squamous cell carcinoma, comparative risk analysis using the Kaplan-Meier method compared by the log-rank test was performed with Bmi-1 amplified and non-amplified groups. All the statistical analyses were conducted with SAS 9.3 software (SAS Institute Inc., Cary, NC).
Immunohistochemical characteristics and analysis of Bmi-1 expression
Comparing the percentage of high Bmi-1 expression in various histologic types
Non-high expression (%)
High expression (%)
Columnar cell metaplasia
Squamous cell carcinoma
Nine of 34 cases of esophageal squamous cell carcinoma and 6 of 80 cases of squamous epithelium showed high expression of Bmi-1 (Figure 3, Table 3). The esophageal squamous cell carcinoma group showed significantly high Bmi-1 expression compared with the squamous epithelium group (p = 0.008).
Correlation of high Bmi-1 expression and clinicopathologic characteristics
The correlation of high Bmi-1 expression with clinicopathologic features was analyzed. High expression of Bmi-1 was significantly associated with poor differentiation in esophageal adenocarcinoma (67%) (Table 2). However, Bmi-1 expression was not associated with age, gender, stage, and lymph node metastasis.
Genomic analysis of Bmi-1 expression
In this study, we reported the first evidence that high expression of Bmi-1 occurred in esophageal adenocarcinoma and its precursor lesions including, low- and high-grade dysplasia, Barrett’s esophagus and columnar cell metaplasia. High Bmi-1 expression increased in intensity and percentage following the early progress of adenocarcinoma from squamous epithelium (7%), columnar cell metaplasia (22%), Barrett’s esophagus (22%), low- (45%) and high-grade dysplasia (43%) and adenocarcinoma (37%). The increase in high Bmi-1 expression was significant from squamous mucosa to columnar cell metaplasia and Barrett’s esophagus and from Barrett’s esophagus to low-grade dysplasia and esophageal adenocarcinoma. In addition, the expression level of Bmi-1 protein was significantly associated with worse histologic grade of esophageal adenocarcinoma. However, high Bmi-1 expression did not show an association with overall survival in both esophageal adenocarcinoma and squamous cell carcinoma. DNA microarray analysis detected a low percentage of Bmi-1 amplification in esophageal adenocarciinoma.
The carcinogenesis of esophageal adenocarcinoma from normal epithelium was suggested to involve a series of events that includes the reflux of gastric and duodenal contents into the esophagus leading to reflux esophagitis, followed by Barrett’s esophagus, dysplasia, and esophageal adenocarcinoma . During these events, the accumulation of genetic and epigenetic aberrations driven by inflammation, as well as acid and bile salt stimulation, contributes to the carcinogenesis. A number of genes including apoptosis-related genes, cell cycle-related genes, tumor suppressor genes, oncogenes and growth factors, have been reported to be involved in esophageal carcinogenesis. Bmi-1 has a role in epigenetic modification as a member of the polycomb-group family, and its abnormal expression may contribute to carcinogenesis . We found Bmi-1 present in the basal layers of normal squamous mucosa layer where the stem cells are usually located, but the percentage of expression was very low and the intensity was weak. However, the percentage and intensity of high Bmi-1 expression were significantly increased following the progression from columnar cell metaplasia to adenocarcinoma. The percentage of high expression cells were doubled in low- and high-grade dysplasia compared with to columnar cell metaplasia and Barrett’s esophagus. Also, the distribution of cells with high Bmi-1 expression was observed to have a different pattern, mostly within the base of columnar cells metaplasia compared with the full gland distribution in dysplasia and adenocarcinoma. The trend of high Bmi-1 expression suggests that Bmi-1 may play an important role in the early carcinogenesis.
From a previous study, Bmi-1 was reported to act as a stable transcriptional repressor that regulates inhibitors of p16INK4a and p19ARF . Our DNA microarray studies lends support to a previous report that p16 and p53 play important roles in early carcinogenesis of esophageal adenocarcinoma . The high expression of Bmi-1 in precancerous lesion implies that Bmi-1 might promote cell proliferation by suppressing p16/Rb and/or p19ARF/MDM2/p53 tumor suppressor pathway in early carcinogenesis. Therefore, further investigation is needed for the role of Bmi-1 in relation to p16, p53, and other signal pathways.
In esophageal adenocarcinoma, the amplification of Bmi-1 was very low (3%) by DNA microarray study compared with to the high expression (37%) by immunohistochemical study. From previous studies on gastric and colonic adenocarcinoma, Bmi-1 was found to upregulate both at the transcriptional and translational levels [18, 20, 21]. We assumed that high Bmi-1 expression may be involved at the transcriptional level without significant DNA amplification, suggesting that the higher expression in esophageal adenocarcinoma is not driven by changes in the DNA copy number. In addition, the 16p segment, where Bmi-1 and several genes are located, is amplified. The worse overall survival may not be solid evidence to prove the association of Bmi-1 amplification with prognosis.
We then analyzed the association between Bmi-1 expression and clinical characteristics of the patients. The analysis revealed a significant correlation between high expression of Bmi-1 in esophageal adenocarcinoma with moderately to poorly differentiated esophageal adenocarcinoma. Unlike previous studies on gastric carcinoma , colonic , and esophageal squamous cell carcinoma , we observed no significant correlation between high expression of Bmi-1 and esophageal adenocarcinoma and squamous cell carcinoma with other clinicopathologic features such as age, gender, stage, metastatic lymph nodes. The difference in the correlation between Bmi-1 and clinicopathologic features may reflect the tissue and population variance of these studies. The previous studies were performed in the Chinese population.
While our study showed high Bmi-1 expression of shows slightly better but not a significant difference in prognosis in esophageal adenocarcinoma, most studies showed that high Bmi-1 expression was associated with poorer prognosis [18–20, 24, 25, 27]. However, Pietersen et al. did report a correlation between high expression of Bmi-1 and better outcome in breast cancer patients . The non-significant prognosis for esophageal adenocarcinoma was unexpected since other gastrointestinal carcinomas showed worse prognosis with high Bmi-1 expression. The different types of epithelium located throughout the gastrointestinal system, molecular mechanisms, and population groups may explain this discrepancy.
In esophageal squamous cell carcinoma, the data on the association between Bmi-1 expression and prognosis are limited and conflicting. Our results for esophageal squamous cell carcinoma are similar to the results from Yamada et al. where they found no association in prognosis between high expression of Bmi-1 and squamous cell carcinoma . In contrast, two other reports found patients with higher expression of Bmi-1 had lower overall survival compared with patients with worse expression of Bmi-1 [28, 29].
In conclusion, Bmi-1 was rarely amplified in esophageal adenocarcinoma, but highly expressed in esophageal adenocarcinoma and squamous cell carcinoma. High expression of Bmi-1 was significantly correlated with the histologic grade of esophageal adenocarcinoma. The accumulation of high Bmi-1 expression from columnar cell metaplasia, Barrett’s esophagus, dysplasia to adenocarcinoma implies an important role of Bmi-1 in the early development of esophageal adenocarcinoma. It also suggests that Bmi-1 is a potential target for treatment of precancerous lesions.
B cell-specific Moloney murine leukemia virus integration site 1
Gastroesophageal reflux disease.
Jun Sun is supported by the NIDDK (KO1 DK075386 and 1R03DK089010-01), the American Cancer Society (RSG-09-075-01-MBC), and the IDEAL award from New York State’s Empire State Stem Cell Board (N09G-279)
This work was supported in part by the National Institute of Health-National Cancer Institute grant 5R01CA090665 (PI: JD Luketich)
We thank Dr. Jorge Yao for constructing the esophageal tissue microarray.
- Kamangar F, Dores GM, Anderson WF: Patterns of cancer incidence, mortality, and prevalence across five continents: defining priorities to reduce cancer disparities in different geographic regions of the world. J Clin Oncol. 2006, 24 (14): 2137-2150. 10.1200/JCO.2005.05.2308.View ArticlePubMedGoogle Scholar
- Parkin DM, Bray F, Ferlay J, Pisani P: Global cancer statistics, 2002. CA Cancer J Clin. 2005, 55 (2): 74-108. 10.3322/canjclin.55.2.74.View ArticlePubMedGoogle Scholar
- Daly JM, Fry WA, Little AG, Winchester DP, McKee RF, Stewart AK, Fremgen AM: Esophageal cancer: results of an American College of Surgeons Patient Care Evaluation Study. J Am Coll Surg. 2000, 190 (5): 562-572. 10.1016/S1072-7515(00)00238-6. discussion 572–563View ArticlePubMedGoogle Scholar
- Vizcaino AP, Moreno V, Lambert R, Parkin DM: Time trends incidence of both major histologic types of esophageal carcinomas in selected countries, 1973–1995. Int J Cancer. 2002, 99 (6): 860-868. 10.1002/ijc.10427.View ArticlePubMedGoogle Scholar
- Howlader N, Noone AM, Krapcho M, Neyman N, Aminou R, Waldron W, Altekruse SF, Kosary CL, Ruhl J, Tatalovich Z, et al: SEER Cancer Statistics Review. 1975, Bethesda, MD: National Cancer Institute, –2008Google Scholar
- Enzinger PC, Mayer RJ: Esophageal cancer. N Engl J Med. 2003, 349 (23): 2241-2252. 10.1056/NEJMra035010.View ArticlePubMedGoogle Scholar
- Chen X, Yang CS: Esophageal adenocarcinoma: a review and perspectives on the mechanism of carcinogenesis and chemoprevention. Carcinogenesis. 2001, 22 (8): 1119-1129. 10.1093/carcin/22.8.1119.View ArticlePubMedGoogle Scholar
- Haupt Y, Alexander WS, Barri G, Klinken SP, Adams JM: Novel zinc finger gene implicated as myc collaborator by retrovirally accelerated lymphomagenesis in E mu-myc transgenic mice. Cell. 1991, 65 (5): 753-763. 10.1016/0092-8674(91)90383-A.View ArticlePubMedGoogle Scholar
- van Lohuizen M, Verbeek S, Scheijen B, Wientjens E, van der Gulden H, Berns A: Identification of cooperating oncogenes in E mu-myc transgenic mice by provirus tagging. Cell. 1991, 65 (5): 737-752. 10.1016/0092-8674(91)90382-9.View ArticlePubMedGoogle Scholar
- Jiang L, Li J, Song L: Bmi-1, stem cells and cancer. Acta Biochim Biophys Sin (Shanghai). 2009, 41 (7): 527-534. 10.1093/abbs/gmp040.View ArticleGoogle Scholar
- van der Lugt NM, Domen J, Linders K, van Roon M, Robanus-Maandag E, te Riele H, van der Valk M, Deschamps J, Sofroniew M, van Lohuizen M, et al: Posterior transformation, neurological abnormalities, and severe hematopoietic defects in mice with a targeted deletion of the bmi-1 proto-oncogene. Genes Dev. 1994, 8 (7): 757-769. 10.1101/gad.8.7.757.View ArticlePubMedGoogle Scholar
- Jacobs JJ, Kieboom K, Marino S, DePinho RA, van Lohuizen M: The oncogene and Polycomb-group gene bmi-1 regulates cell proliferation and senescence through the ink4a locus. Nature. 1999, 397 (6715): 164-168. 10.1038/16476.View ArticlePubMedGoogle Scholar
- Jacobs JJ, Scheijen B, Voncken JW, Kieboom K, Berns A, van Lohuizen M: Bmi-1 collaborates with c-Myc in tumorigenesis by inhibiting c-Myc-induced apoptosis via INK4a/ARF. Genes Dev. 1999, 13 (20): 2678-2690. 10.1101/gad.13.20.2678.View ArticlePubMedPubMed CentralGoogle Scholar
- Satijn DP, Otte AP: RING1 interacts with multiple Polycomb-group proteins and displays tumorigenic activity. Mol Cell Biol. 1999, 19 (1): 57-68.View ArticlePubMedPubMed CentralGoogle Scholar
- Bea S, Tort F, Pinyol M, Puig X, Hernandez L, Hernandez S, Fernandez PL, van Lohuizen M, Colomer D, Campo E: BMI-1 gene amplification and overexpression in hematological malignancies occur mainly in mantle cell lymphomas. Cancer Res. 2001, 61 (6): 2409-2412.PubMedGoogle Scholar
- Raaphorst FM, van Kemenade FJ, Blokzijl T, Fieret E, Hamer KM, Satijn DP, Otte AP, Meijer CJ: Coexpression of BMI-1 and EZH2 polycomb group genes in Reed-Sternberg cells of Hodgkin’s disease. Am J Pathol. 2000, 157 (3): 709-715. 10.1016/S0002-9440(10)64583-X.View ArticlePubMedPubMed CentralGoogle Scholar
- van Kemenade FJ, Raaphorst FM, Blokzijl T, Fieret E, Hamer KM, Satijn DP, Otte AP, Meijer CJ: Coexpression of BMI-1 and EZH2 polycomb-group proteins is associated with cycling cells and degree of malignancy in B-cell non-Hodgkin lymphoma. Blood. 2001, 97 (12): 3896-3901. 10.1182/blood.V97.12.3896.View ArticlePubMedGoogle Scholar
- Liu JH, Song LB, Zhang X, Guo BH, Feng Y, Li XX, Liao WT, Zeng MS, Huang KH: Bmi-1 expression predicts prognosis for patients with gastric carcinoma. J Surg Oncol. 2008, 97 (3): 267-272. 10.1002/jso.20934.View ArticlePubMedGoogle Scholar
- Yonemitsu Y, Imazeki F, Chiba T, Fukai K, Nagai Y, Miyagi S, Arai M, Aoki R, Miyazaki M, Nakatani Y, et al: Distinct expression of polycomb group proteins EZH2 and BMI1 in hepatocellular carcinoma. Hum Pathol. 2009, 40 (9): 1304-1311. 10.1016/j.humpath.2009.01.017.View ArticlePubMedGoogle Scholar
- Li DW, Tang HM, Fan JW, Yan DW, Zhou CZ, Li SX, Wang XL, Peng ZH: Expression level of Bmi-1 oncoprotein is associated with progression and prognosis in colon cancer. J Cancer Res Clin Oncol. 2010, 136 (7): 997-1006. 10.1007/s00432-009-0745-7.View ArticlePubMedGoogle Scholar
- Kim JH, Yoon SY, Kim CN, Joo JH, Moon SK, Choe IS, Choe YK, Kim JW: The Bmi-1 oncoprotein is overexpressed in human colorectal cancer and correlates with the reduced p16INK4a/p14ARF proteins. Cancer Lett. 2004, 203 (2): 217-224. 10.1016/j.canlet.2003.07.009.View ArticlePubMedGoogle Scholar
- Pietersen AM, Horlings HM, Hauptmann M, Langerod A, Ajouaou A, Cornelissen-Steijger P, Wessels LF, Jonkers J, van de Vijver MJ, van Lohuizen M: EZH2 and BMI1 inversely correlate with prognosis and TP53 mutation in breast cancer. Breast Cancer Res. 2008, 10 (6): R109-View ArticlePubMedPubMed CentralGoogle Scholar
- Kim JH, Yoon SY, Jeong SH, Kim SY, Moon SK, Joo JH, Lee Y, Choe IS, Kim JW: Overexpression of Bmi-1 oncoprotein correlates with axillary lymph node metastases in invasive ductal breast cancer. Breast. 2004, 13 (5): 383-388. 10.1016/j.breast.2004.02.010.View ArticlePubMedGoogle Scholar
- Qin ZK, Yang JA, Ye YL, Zhang X, Xu LH, Zhou FJ, Han H, Liu ZW, Song LB, Zeng MS: Expression of Bmi-1 is a prognostic marker in bladder cancer. BMC Cancer. 2009, 9: 61-10.1186/1471-2407-9-61.View ArticlePubMedPubMed CentralGoogle Scholar
- Song LB, Zeng MS, Liao WT, Zhang L, Mo HY, Liu WL, Shao JY, Wu QL, Li MZ, Xia YF, et al: Bmi-1 is a novel molecular marker of nasopharyngeal carcinoma progression and immortalizes primary human nasopharyngeal epithelial cells. Cancer Res. 2006, 66 (12): 6225-6232. 10.1158/0008-5472.CAN-06-0094.View ArticlePubMedGoogle Scholar
- Kang MK, Kim RH, Kim SJ, Yip FK, Shin KH, Dimri GP, Christensen R, Han T, Park NH: Elevated Bmi-1 expression is associated with dysplastic cell transformation during oral carcinogenesis and is required for cancer cell replication and survival. Br J Cancer. 2007, 96 (1): 126-133. 10.1038/sj.bjc.6603529.View ArticlePubMedGoogle Scholar
- Vonlanthen S, Heighway J, Altermatt HJ, Gugger M, Kappeler A, Borner MM, van Lohuizen M, Betticher DC: The bmi-1 oncoprotein is differentially expressed in non-small cell lung cancer and correlates with INK4A-ARF locus expression. Br J Cancer. 2001, 84 (10): 1372-1376. 10.1054/bjoc.2001.1791.View ArticlePubMedPubMed CentralGoogle Scholar
- He XT, Cao XF, Ji L, Zhu B, Lv J, Wang DD, Lu PH, Cui HG: Association between Bmi1 and clinicopathological status of esophageal squamous cell carcinoma. World J Gastroenterol. 2009, 15 (19): 2389-2394. 10.3748/wjg.15.2389.View ArticlePubMedPubMed CentralGoogle Scholar
- Liu WL, Guo XZ, Zhang LJ, Wang JY, Zhang G, Guan S, Chen YM, Kong QL, Xu LH, Li MZ, et al: Prognostic relevance of Bmi-1 expression and autoantibodies in esophageal squamous cell carcinoma. BMC Cancer. 2010, 10: 467-10.1186/1471-2407-10-467.View ArticlePubMedPubMed CentralGoogle Scholar
- Yamada A, Fujii S, Daiko H, Nishimura M, Chiba T, Ochiai A: Aberrant expression of EZH2 is associated with a poor outcome and P53 alteration in squamous cell carcinoma of the esophagus. Int J Oncol. 2011, 38 (2): 345-353.PubMedGoogle Scholar
- Dulak AM, Schumacher SE, van Lieshout J, Imamura Y, Fox C, Shim B, Ramos AH, Saksena G, Baca SC, Baselga J, et al: Gastrointestinal Adenocarcinomas of the Esophagus, Stomach, and Colon Exhibit Distinct Patterns of Genome Instability and Oncogenesis. Cancer Res. 2012, 72 (17): 4383-4393. 10.1158/0008-5472.CAN-11-3893.View ArticlePubMedPubMed CentralGoogle Scholar
- Cohen KJ, Hanna JS, Prescott JE, Dang CV: Transformation by the Bmi-1 oncoprotein correlates with its subnuclear localization but not its transcriptional suppression activity. Mol Cell Biol. 1996, 16 (10): 5527-5535.View ArticlePubMedPubMed CentralGoogle Scholar
- Reinisch CM, Uthman A, Erovic BM, Pammer J: Expression of BMI-1 in normal skin and inflammatory and neoplastic skin lesions. J Cutan Pathol. 2007, 34 (2): 174-180. 10.1111/j.1600-0560.2006.00587.x.View ArticlePubMedGoogle Scholar
- Kim TH, Lee HM, Lee SH, Choe H, Kim HK, Lee JH, Oh KH: Expression and distribution patterns of the stem cell marker, nestin, and the stem cell renewal factor, BMI-1, in normal human nasal mucosa and nasal polyps. Acta Otolaryngol. 2009, 129 (9): 996-1001. 10.1080/00016480802527560.View ArticlePubMedGoogle Scholar
- Valk-Lingbeek ME, Bruggeman SW, van Lohuizen M: Stem cells and cancer; the polycomb connection. Cell. 2004, 118 (4): 409-418. 10.1016/j.cell.2004.08.005.View ArticlePubMedGoogle Scholar
- Langer R, Von Rahden BH, Nahrig J, Von Weyhern C, Reiter R, Feith M, Stein HJ, Siewert JR, Hofler H, Sarbia M: Prognostic significance of expression patterns of c-erbB-2, p53, p16INK4A, p27KIP1, cyclin D1 and epidermal growth factor receptor in oesophageal adenocarcinoma: a tissue microarray study. J Clin Pathol. 2006, 59 (6): 631-634. 10.1136/jcp.2005.034298.View ArticlePubMedPubMed CentralGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-230X/12/146/prepub
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