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Fig. 4 | BMC Gastroenterology

Fig. 4

From: Uridine stimulate laxative effect in the loperamide-induced constipation of SD rats through regulation of the mAChRs signaling pathway and mucin secretion

Fig. 4

Expression of key proteins in the mAChR M2 and M3 downstream signaling pathway. The expression of several related proteins including PKC, p-PKC, PI3K and p-PI3K was measured by Western blot analysis using HRP-labeled anti-rabbit IgG antibody. After the intensity of each band was determined using an imaging densitometer, the relative levels of four proteins were calculated based on the intensity of actin protein. Five to six rats per group were assayed in triplicate by Western blotting. Data represent the means ± SD of three replicates. *, p < 0.05 compared to the No treated group. #, p < 0.05 compared to the Lop + Vehicle treated group

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BMC Gastroenterology

ISSN: 1471-230X

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