Fig. 1
Adhesion time analysis of GTL-16. In a on the left, optical microscopy images of adherent GTL-16 taken at original magnification 20X. On the right, the number of adherent cells for each treatment is reported. The ratio reports a mean ± (SD) (%) of adherent cells counted in 12 different areas. Data reported are means of 5 biological replicates. G = Grisù®; G + V = co-stimulation with Grisù® and vitD3. b, c, d p < 0.05 vs a; a, f, e p < 0.05 vs d; e p < 0.05 vs b; f p < 0.05 vs c. In b on the left, optical microscopy images of the cells treated with Grisù® alone or in combination with vitD3 after 2 min and 90 min are shown. The scale reported in the first picture is to be considered valid for all images. On the right, the cell counting of adherent cells during time (at 2 min, 5 min, 15 min, 30 min, 60 min, 90 min, 240 min, 720 min) is reported. The ratio reports a mean ± (SD) (%) of adherent cells counted in 12 different areas of 5 biological replicates. The abbreviations are the same reported in a. All samples are significant p < 0.05 vs control