Table 2 Histological scores of livers by using NAFLD activity score (NAS) 17)
10 W model | 48 W model | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
Item | Definition | Score | iNOS+/+ | iNOS−/− | iNOS+/+ | iNOS−/− | iNOS+/+ | iNOS−/− | iNOS+/+ | iNOS−/− |
BD | BD | HFD | HFD | BD | BD | HFD | HFD | |||
Steatosis | ||||||||||
Grade | Parenchymal involvement | |||||||||
<5% | 0 | 6 | 6 | 0 | 0 | 6 | 6 | 0 | 0 | |
5-33% | 1 | 0 | 0 | 4 | 6 | 0 | 0 | 3 | 6 | |
33-66% | 2 | 0 | 0 | 2 | 0 | 0 | 0 | 3 | 0 | |
>66% | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
Average | 0.00 | 0.00 | 1.33 | 1.00 | 0.00 | 0.00 | 1.50 | 1.00 | ||
Inflammation | ||||||||||
Lobular inflammation | Assessment of all inflammatory foci | |||||||||
No foci | 0 | 6 | 6 | 5 | 4 | 6 | 6 | 0 | 0 | |
<2 foci per 200 × field | 1 | 0 | 0 | 1 | 3 | 0 | 0 | 5 | 2 | |
2-4 foci per 200 × field | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | |
>4foci per 200 × field | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
Liver cell injury | Ballooning | |||||||||
None | 0 | 6 | 6 | 0 | 0 | 6 | 6 | 0 | 0 | |
Few balloon cells | 1 | 0 | 0 | 6 | 6 | 0 | 0 | 4 | 0 | |
Many cells/prominent balloooning | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 6 | |
Average | 0.00 | 0.00 | 1.17 | 1.33 | 0.00 | 0.00 | 2.50* | 3.67* | ||
Fibrosis | ||||||||||
Stage | Method of Brunt | |||||||||
None | 0 | 6 | 6 | 6 | 6 | 6 | 6 | 0 | 0 | |
Perivenular/perisinusoidal fibrosis | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 0 | |
Combine pericellular portal fibrosis | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | |
Septal/bridging fibrosis | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | |
Cirrhosis | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
Average | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 1.00** | 2.17** | ||