Figure 4
From: Congenital lactose intolerance is triggered by severe mutations on both alleles of the lactase gene
Biosynthesis and glycosylation pattern of wild type LPH, LPH-Y1473X and LPH-D1796fs at 20°C and 37°C. Transiently transfected COS-1 cells, expressing wild type LPH, LPH-Y1473X or LPH-D1796fs were labeled with [35S] methionine for 3 h and chased for 6 h and 18 h at 20°C (A) and 37°C (B) followed by cell lysis, immunoprecipitation and endo H treatment. The proteins were subjected to SDS-PAGE and autoradiography. The quantification was performed by Quantity One® software.