Figure 4From: Hepatitis B virus inhibition in mice by lentiviral vector mediated short hairpin RNA The inhibitory effects on HBV RNA levels by transfection with HBV ShRNAs. NOD SCID mice were cotransfected with 12 μg pTHBV2 and 1.6-2.0 ml pWPT-HBV- ShRNA-1, 2 or negative control shRNA. The mice were sacrificed 4 d and 7 d after cotransfection, and hepatocytes RNA was extracted. Each experiment was carried out in triplicate. (A) The total levels of HBV S-mRNA in NOD SCID mouse livers were significantly reduced on day 4 or 7 after treatment with pWPT- HBV-shRNA-1 and shRNA-2 lentivirus. (B) The total levels of HBV C-mRNA were significantly decreased on day 4 or 7 after treatment with pWPT-HBV-shRNAs in mice. (error bars indicate standard errors). The values shown were the average of three experiments (means standard deviation). The mean value of empty control was defined as 100%. * P < 0.05 vs. the empty vector group. # P > 0.05 vs. the empty vector group.Back to article page