Figure 1From: Hepatitis B virus inhibition in mice by lentiviral vector mediated short hairpin RNA Target site and vector information. (A) Location of RNAi target sites. Downward arrows indicate the location of RNAi target sites within the four HBV transcripts. The 3.5-kb transcript is the pregenomic RNA that serves as the template for HBV viral DNA replication. The HBV open reading frames are shown below aligned with the HBV mRNAs. Pol: polymerase; Core: HBcAg; S1: large pre-surface antigen; S2: middle pre-surface antigen; S: HBsAg; X: X gene. (B) The construction schematic of lentiviral vector expressing shRNAs. The shRNA expression cassette, including the U6 pol III promoter, the sense and antisense sequence of the shRNA separated by a 8-base loop, and a terminator composed of six thymidines is inserted directly upstream of the CMV promoter of EGFP in the pWPT-GFP vector. Arrows indicate the orientation of transcription for a given gene. The putative HBVshRNA-1 and HBVshRNA-2 structures are shown at the bottom.Back to article page