Training Group | Validation Group | Significance p training vs validation included | |||||
---|---|---|---|---|---|---|---|
Characteristic | Included N = 160 | Non-included N = 78 | Significance p | Included N = 97 | Non-included N = 69 | Significance p | |
Demographics mean (se)or n (%) | |||||||
Age at biopsy, years | 52.9 (0.89) | 54.2 (1.37) | 0.34 | 48.5 (1.3) | 49.1 (1.5) | 0.72 | 0.01 |
Male | 96 (60) | 47 (60) | 0.36 | 57 (59) | 38 (69) | 0.64 | 0.84 |
Height, meter | 1.68 (0.01) | 1.66 (0.01) | 0.20 | 1.69 (0.01) | 1.67 (0.01) | 0.27 | 0.81 |
Weight, kg | 83.6 (1.2) | 76.3 (2.6) | 0.01 | 77.4 (1.5) | 74.0 (1.9) | 0.04 | 0.002 |
Body Mass Index, kg/m2 | 29.6 (0.4) | 27.6 (0.9) | 0.03 | 27.1 (0.5) | 26.4 (0.6) | 0.11 | < 0.0001 |
Metabolic factor (%) | |||||||
Diabetes | 65/160 (41) | 12/78 (15) | < 0.0001 | 31/97 (32) | 15/69 (22) | 0.62 | 0.0002 |
Arterial Hypertension | 53/160 (33) | 6/23 (26) | 15/97 (16) | 16/69 (23) | 0.21 | 0.002 | |
Body Mass Index greater than 27 kg/m2 | 101/160 (63) | 16/34 (47) | 43/97 (44) | 22/69 (32) | 0.11 | 0.003 | |
Triglycerides greater than 1.5 | 70/160 (44) | 20/54 (37) | 34/97 (35) | 15/69 (22) | 0.06 | 0.17 | |
HDL cholesterol lower than 40 mg/L | 36/151 (24) | 6/38 (16) | 15/94 (16) | 15/66 (23) | 0.28 | 0.14 | |
Metabolic factor | |||||||
None | 1/160 (1) | 22/78 (28) | 2/97 (2) | 0/69 | 0.23 | 0.30 | |
At least one | 159/160 (99) | 56/78 (72) | 95/97 (98) | 69/69 | |||
Alcohol consumption, n (%) | |||||||
No | 114/160 (71) | 61/78 (78) | 0.25 | 57/97 (59) | 41/69 (59) | ||
Yes | 46/160 (29) | 17/78 (22) | 40/97 (41) | 28/69 (41) | 0.63 | 0.04 | |
Fibrosis stage, n (%) | |||||||
Non advanced fibrosis | 121/160 (76) | 71/75 (95) | 0.0001 | 66/97(68) | 51/69(74) | 0.48 | 0.12 |
No fibrosis | 69/160 (41) | 50/75 (67) | 25/97 (27) | 21/69 (30) | |||
Fibrosis perisinusoidal or periportal | 52/160 (33) | 21/75 (28) | 41/97 (41) | 30/69 (43) | |||
A: mild, zone 3, perisinusoidal | 29/160 (18) | 19/75 (25) | 16/97 (19) | 18/69 (25) | |||
B: moderate, zone 3, perisinusoidal | 1/160 (1) | 2/75 (3) | 15/97 (15) | 0/69 (0) | |||
C: portal/periportal | 22/160 (14) | 0/75 (0) | 10/97 (22) | 12/69 (18) | |||
Advanced Fibrosis | 39/160 (24) | 4/75 (3) | 31/97 (32) | 18/69(26) | |||
Perisinusoidal and portal/periportal | 20/160 (13) | 1/75(1) | 15/97 (15) | 13/69 (19) | |||
Bridging | 10/160 (6) | 1/75 (1) | 12/97 (12) | 2/69 (3) | |||
Cirrhosis | 9/160 (6) | 2/75 (3) | 4/97 (4) | 3/69 (4) | |||
Non alcoholo-steato-hepatitis (Nash), n (%) | |||||||
No | 57/160 (36) | 39/75 (52) | 0.0001 | 41/97 (42) | NA | NA | 0.04 |
Borderline | 75/160 (47) | 27/75 (36) | 21/97 (22) | NA | |||
Yes | 28/160 (17) | 9/75 (12) | 35/97 (36) | NA | |||
Steatosis | |||||||
< 5% | 10/160 (6) | 24/75 (32)* | 0.0001 | 19/97 (20)** | 13/69 (19) | 0.38 | < 0.0001 |
5–32 | 56/160 (35) | 16/75 (21) | 22/97 (23) | 27/69 (39) | |||
33–65 | 46/160 (29) | 16/75 (21) | 27/97 (28) | 10/69 (14) | |||
66–100 | 48/160 (30) | 19/75 (25) | 29/97 (30) | 19/60 (28) | |||
Interval between biopsy and serum, median (95%CI), Range days | 0[0; 1] -87;+89 | 0[-4; 1] -538; +540 | < 0.0001 | 0[0; 0] -24;+49 | 0[0; 0] -42; +40 | 0.90 | 0.07 |
Biopsy quality mean (se) | |||||||
Sample size | 20.1 (0.5) | 19.3 (0.8) | 0.33 | 17.7 (0.7) | 21.4 (0.9) | 0.001 | 0.002 |
Number of fragment | 2.2 (0.2) | 2.4 (0.2) | 0.88 | 2.3 (0.2) | 2.2 (0.2) | 0.79 | 0.61 |
Number of portal tracts | 16.4 (0.6) | 15.5 (0.9) | 0.28 | 13.6 (0.6) | 17.1 (1.0) | 0.008 | 0.01 |
Serum biochemical markers, mean (se) | |||||||
α2-macroglobulin (g/L) | 1.70 (0.05) | 1.70 (0.10) | 0.74 | 1.72 (0.06) | 1.76 (0.08) | 0.47 | 0.50 |
ALT (IU) | 72 (3) | 63 (5) | 0.10 | 79 (5) | 84 (10) | 0.69 | 0.35 |
AST (IU) † | 50 (3) | 44 (3) | 0.10 | 45 (3) | 52 (5) | 0.05 | 0.006 |
Apolipoprotein A1(g/L) | 1.47 (0.02) | 1.45 (0.06) | 0.98 | 1.59 (0.07) | 1.53 (0.04) | 0.80 | 0.06 |
GGT (IU/L) | 111 (12) | 108 (15) | 0.83 | 132 (12) | 158 (19) | 0.16 | 0.04 |
Haptoglobin (g/L) | 1.09 (0.04) | 1.18 (0.11) | 0.41 | 1.12 (0.10) | 1.02 (0.05) | 0.70 | 0.39 |
Total bilirubin (μmol/L) | 11.4 (0.5) | 22.1 (9.5) | 0.35 | 12.6 (0.6) | 13.6 (1.0) | 0.89 | 0.02 |
Glucose (mmol/L) | 6.1 (0.2) | 5.3 (0.2) | 0.08 | 6.1 (0.2) | 5.8 (0.3) | 0.008 | 0.46 |
Cholesterol (mmol/L) | 5.5 (0.1) | 5.5 (0.2) | 0.87 | 5.4 (0.1) | 5.5 (0.1) | 0.57 | 0.84 |
Triglycerides (mmol/L) | 2.0 (0.1) | 1.6 (0.1) | 0.31 | 1.4 (0.1) | 1.3 (0.1) | 0.03 | 0.04 |
FibroTest (0.00–1.00) | 0.30 (0.02) | 0.35 (0.05) | 0.59 | 0.29 (0.02) | 0.33 (0.02) | 0.21 | 0.99 |
SteatoTest (0.00–1.00) | 0.70 (0.01) | 0.56 (0.05) | 0.007 | 0.61 (0.03) | 0.60 (0.03) | 0.54 | 0.02 |