Table 1 Primers used in real-time PCR to quantify levels of mRNA expression.

IFNγ

Forward: 5'- GGCTGTCCCTGAAAGAAAGC -3'

Reverse: 5'- GAGCGAGTTATTTGTCATTCGG -3'

TNFα

Forward: 5'- AAGGGAGAGTGGTCAGGTTGCC -3'

Reverse: 5'- CCTCAGGGAAGAGTCTGGAAAGG -3'

IFNα

Forward: 5'- GACTTTGGATTTCCCCTGGAG -3'

Reverse: 5'- AAGCCTTTGATGTGAAGAGGTTC -3'

IFNβ

Forward: 5'- GTTACACTGCCTTTGCCATCC -3'

Reverse: 5'- CAACAATAGTCTCATTCCACCCAG -3'

IL-4

Forward: 5'- CCACGGATGCGACAAAAATC

Reverse: 5'- TGTTCTTCGTTGCTGTGAGGAC

IL-5

Forward: 5'- TCCCTGCTACTCTCCCCAAAC

Reverse: 5'- TGGCACAGTCTGATTCATACATAGG

IL-18

Forward: 5'- AAATGGAGACCTGGAATCAGAC -3'

Reverse: 5'- TTTGTCAACGAAGAGAACTTGG -3'

IL-23

Forward: 5'-CCCGTATCCAGTGTGAAGATG -3'

Reverse: 5'- TGTCAGAGTCAAGCAGGTGC -3'

IL-27

Forward: 5'- TGTTCAAAGGAGGAGGAGGAC -3'

Reverse: 5'- GGATGACACCTGATTGGGG -3'

Rotavirus VP6

Forward: 5'- GCGGTAGCGGTGTTATTTCC -3'

Reverse: 5'- TTGTTTTGCTTGCGTCGG -3'

Rotavirus NSP3

Forward: 5'- TGTCAAGAGAATACCTGGGAAATC -3'

Reverse: 5'- GGAATCATCAACTTCAACTTCACC -3'

GAPDH

Forward: 5'- TGGTTTGACAATGAATACGGCTAC

Reverse: 5'- GGTGGGTGGTCCAAGGTTTC