Figure 1

Outcome of IL-12KO mice infected with RRV. (A) Injection of RRV into newborn Balb/c mice leads to growth failure, as demonstrated by a significantly lower body weight when compared to non-infected mice (P < 0.05). RRV injection also leads to symptoms of cholestasis in mice between 4–8 days (B), and to decreased survival (C). The poor growth, onset of cholestasis, and decreased survival are similar to wild-type (WT) mice infected with RRV. Total number of animals at the time of injection of RRV or saline: IL-12KO-saline = 8, IL-12KO-RRV = 21, WT-RRV = 19. During the course of the study, all mice injected with normal saline remained healthy. In RRV injected IL-12KO mice, 20 out of 21 animal had died by day 17; similarly, 17 out of 19 RRV infected WT mice had died by day 21.