Figure 5
From: Long-term culture of cholangiocytes from liver fibro-granulomatous lesions
Homogeneous cultures of ductal epithelial cells. A. Phase contrast showing a homogeneous culture of epithelial cells. (750×). B. Comparative flow cytometry analysis of epithelial-enriched (red line) and mixed populations (blue line). X axis, Forward-angle light scatter representing cell size; Y axis, relative cell number. C. Immunofluorescence for cytokeratin-18 (600×). D. Immunofluorescence for cytokeratin-19 (600×) E. Negative control for immunofluorescence incubated with an irrelevant antibody (600×).F. Tubules of isolated cultures. Single-cell suspensions were allowed to proliferate. A previous layer of gel was made containing liver connective tissue cells as a feeder layer. Phase contrast microscopy (125×).