Figure 1

CYP3A5, CYP2E1, CYP3A4, and CYP2C mRNA expression in colon mucosa of patients with adenoma and disease-free controls. (A) Representative agarose gel of mRNA integrity. Lane 1 and 12 = positive control, lane 2–11 = RNA extracted from colonic mucosa biopsies. (B) Representative photomicrograph of RT-PCR products of two subjects. All measurements were carried out in triplicate. Lanes 1, 6, 11: Histone 3.3; Lanes 2, 7, 12: CYP3A5; Lanes 3, 8, 13: CYP2E1; Lanes 4, 9, 14: CYP3A4; Lanes 5, 10, 15 = CYP2C, bp = base pairs. (C) Quantitative analysis of CYP2C, CYP2E1, CYP3A4, and CYP3A5 mRNA expression in normal colon mucosa and in colonic adenoma of patients. Results are normalized to histone 3.3 expression. Data are means ± SEM, ^ap < 0.05 compared to normal tissue. (D) Quantitative analysis of CYP2C, CYP2E1, CYP3A4, and CYP3A5 mRNA expression in normal colon mucosa of patients with colonic adenoma and disease-free controls. Results are normalized to histone 3.3 expression. Data are means ± SEM, ^ap < 0.05 compared to disease-free controls.