Figure 1

A. Expression of KIT gene using semi-quantitative RT-PCR, in gastric fundic tissues from wild type and W/W ^Vmutant mice maintained under fasted conditions. GAPDH levels were measured as a control. B. Representative image of DNA microarray hybridization. Alterations in gene expression that were represented by the ratio of intensity of fluorescence measured using Cy3 and Cy5 fluorochromes. Expression profiles were evaluated by reverse transcription of poly (A)+ RNAs from gastric fundic muscles from wild type and W/W ^Vmice in the presence of Cy3 or Cy5 fluorescent labeling dyes followed by hybridization to mouse GEM 1 microarrays. The image shown was produced by superimposing the Cy5 fluorescence image (pseudo-coloured green that represents expression level of RNA from the tunica muscularis of W/W ^Vfundus) and the Cy3 fluorescence image (pseudo-coloured red that represents RNA expression from wild type tissues). Arrays were counterstained with DAPI (blue). C. Confirmation of the reliability of the microarray data by semi-quantitative RT-PCR. Representative examples of RT-PCR analysis, in fundic tissues from six wild type and six W/W ^Vmutant mice maintained under starved conditions. Expression of the CPO gene was considerably reduced in the fundus of W/W ^Vmice compared to age matched wild type mice, whereas the MCM7 gene showed an increase in expression in W/W ^Vmice. D. Relative expression level of CPO (CPO/GAPDH) and MCM7 (MCM7/GAPDH) in fundic tissues from six wild type and six W/W ^Vmutant mice. The data represent the mean ( ± SD) of six different RT-PCR experiments using six wild type or W/W ^V fundus RNA as templates.