Figure 1

Expression of NPM1 in gastric samples. (A) protein expression normalized by ACTB and calibrated by the level of a reference sample; (B) the ratio of NPM1 protein expression between tumor and matched non-neoplastic samples; (C) Western blot using anti-NPM1 and anti-ACTB antibodies in representative gastric tumors and their matched non-neoplastic counterparts; (D) mRNA expression normalized by ACTB and calibrated by a non-neoplastic gastric tissue; (E) relative quantification of NPM1 mRNA expression, in which gastric tumors samples were calibrated by the matched non-neoplastic tissue; (F) relationship between the relative quantification of NPM1 protein and mRNA levels, in which non-neoplastic samples were used as reference for each matched tumor. T: tumor samples; N: non-neoplastic samples; RQ: Relative quantification. *Reference sample in Western blot gels.