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Table 1 Zinc serum levels and jejunal morphometrics in experimental rats challenged by lactose-induced diarrhea and the regional northeast basic diet with their respective controls

From: Zinc treatment ameliorates diarrhea and intestinal inflammation in undernourished rats

 

Nourished

Undernourished

Outcomes

PBS

PBS

P

Zn

P (Zn vs PBS)

Lac

Zn + lac

P (Zn + Lac vs Lac)

Biochemisty

        

Zinc serum levels (mg/L) (n)

1.089 ± 0.08 (n = 11)

0.712 ± 0.10 (n = 10)

< 0.0001

1.57 ± 0.12 (n = 8)

< 0.0001

1.12 ± 0.24 (n = 12)

2.04 ± 0.50 (n = 8)

< 0.0001

Jejunal morphology*

        

Villus height (μm)

826.3 ± 92.2

587.9 ± 98.3

< 0.0001

704.2 ± 113.9

0.001

686 ± 103.2

667.9 ± 78.2

0.359

Crypt length (μm)

151.5 ± 36.5

187.7 ± 66.7

< 0.0001

169.8 ± 49.0

0.007

141.6 ± 27.7

127.2 ± 20.3

< 0.0001

Villus/crypt ratio

6.03 ± 1.7

3.7 ± 1.4

< 0.0001

5.15 ± 2.82

0.0322

5.1 ± 0.8

5.4 ± 1.19

0.235

  1. *At least 40 sections of jejunal villi and crypts stained with hematoxylin and eosin in at least four animals per group. Data are presented as mean ± SD. Comparisons were performed by Student’s unpaired t test. Villi and crypts were measured only when their full longitudinal axis was found.
  2. Zn = zinc treated-rats; Lac = lactose challenged-rats.