Table 1 Primer sequences used for QRT-PCR
From: Role of the ABCG8 19H risk allele in cholesterol absorption and gallstone disease
Fragment | Primer | Sequence | Product size |
|---|---|---|---|
Exon 9/10 | ABCG5 F | 5’ – CGCGTAGGTCTCCTTTACCA – 3’ | 152 |
|  | ABCG5 R | 5’ – AGTGCATAGGCCAGCATCAT – 3’ |  |
Exon 10/11 | ABCG8 F | 5’ – GTGGCTGGTGGTCTTCTGTT – 3’ | 84 |
|  | ABCG8 R | 5’ – CTGAAGAAGGAGGCCATGTG – 3’ |  |
Exon 17/18 | NPC1L1 F | 5’ – GTGGGGCATCAGTTACAATG – 3’ | 166 |
|  | NPC1L1 R | 5’ – AAACACCGCACTTCCCATAG – 3’ |  |
Exon 4/5 | LXRα F | 5’ – TCAGGCGGATCTGTTCTTCT – 3’ | 213 |
|  | LXRα R | 5’ – TCAGGCGGATCTGTTCTTCT – 3’ |  |
Exon 7/8 | LXRβ F | 5’ – TAAGCAAGTGCCTGGTTTCC – 3’ | 200 |
|  | LXRβ R | 5’ – ACTCGAAGATGGGGTTGATG – 3’ |  |
Exon 8/10 | HNF1α F | 5’ – CACGCCCACCAAGCAGGTCT – 3’ | 165 |
|  | HNF1α R | 5’ – CCAGGCTGCTGGAGGACACTG – 3’ |  |
Exon 9/10 | HNF4α F | 5’ – ATGCTTCCGGGCTGGC – 3’ | 235 |
|  | HNF4α R | 5’ – TTCGAGTGCTGATCCG – 3’ |  |
Exon 4/5 | SREB2 F | 5’ – CAGCCTCAGATCATCAAGACA – 3’ | 202 |
|  | SREB2 R | 5’ – CTTTCTCTTGCCCCATCATTA – 3’ |  |
Exon 4/5 | PPARδ F | 5’ – ATGGAGCAGCCACAGGAGGAAGCC – 3’ | 200 |
|  | PPARδ R | 5’ – GCATGAGGCCCCGTCACAGC – 3’ |  |