Figure 2

Berberine reduces proliferation and induced apoptosis in intestinal tumors of Apc ^min/+ mice. (A-B) Distal small intestinal sections from untreated and 0.1% berberine-treated mice were stained with PCNA and Ki-67. (C-D) Small intestinal segments from untreated and 0.1% berberine-treated groups were processed for TUNEL and cleaved caspase-3 staining (400×). Data were quantified as mean percentage of positive cells at five randomly selected fields in each sample. Scale bars, 50 μm. Columns, means from at least six mice in each group; bars, standard deviation. *, P < 0.01, 0.1% berberine-treated vs untreated Apc ^min/+ mice.