Figure 4

Quantitative Real-time PCR and western blot analysis of TNF- α, MCP-1, TGF-β, PDGF-β, Shh, GLI1 and α-SMA mRNA and protein expression in the pancreas of control mice and caerulein induced chronic pancreatitis mice with or without sulindac treatment. (A) TNF-α expression; (B) MCP-1 expression, (C) TGF-β expression, (D) PDGF-β expression, (E) SHH expression, (F) GLI1 expression and (G) α-SMA expression (*: Statistic difference, p < 0.05). (H) Western blot results of TNF-α, MCP-1, TGF-β, PDGF-β, SHH, GLI1 and α-SMA expression, using β-actin as loading control.