Effect of a multispecies probiotic supplement on quantity of irritable bowel syndrome-related intestinal microbial phylotypes

Table 2 qPCR primers and assay conditions.

Assay	Primers (5' → 3')	Standard	Classification of standard ( > 98% unless otherwise stated)	Target size (bp)	MgCl₂ (mM)	Annealing T (°C)	Detection T (°C)
Bacteroides intestinalis-like [11]	F: AGCATGACCTAGCAATAGGTT R: CCTTCTCGTTATACTATCCGGTAT	[EMBL:AM277809]	Bacteroides	124	3	63	83
Clostridium cocleatum 88% [9]	F: AATACATAAGTAACCTGGCRTC R: CGTAGCACTTTTCATATAGAGTT	[EMBL:AM276544]	Erysipelotrichaceae	104	4	60	80
Clostridium thermosuccinogenes 85% [11]	F: ACATGCAAGTCGAACGGAAGTC R: TGCGTCAGAGTTTCCTCCATTG	[EMBL:AM275406]	Clostridiales 97%	373	2	62	81
Collinsella aerofaciens-like [9]	F: CCCGACGGGAGGGGAT R: CTTCTGCAGGTACAGTCTTGAC	[EMBL:AM276090]	Collinsella	260	4	67	89
Coprococcus eutactus-like [9]	F: AGCTTGCTCCGGCYGATTTA R: CGGTTTTACCAGTCGTTTCCAA	[EMBL:AM275825]	Coprococcus	97	2	63	83
Ruminococcus torques 91% [9]	F: TGCTTAACTGATCTTCTTCGGA R: CGGTATTAGCAGTCATTTCTG	[EMBL:AM276624]	Lachnospiraceae	119	5	62	82
Ruminococcus torques 93% [11]	F: GACTGCTTTTGAAACTGTCA R: AGGTCCGGTTAAGGA	[EMBL:AM275798]	Lachnospiraceae	396	4	61	83
Ruminococcus torques 94% [9]	F: AATCTTCGGAGGAAGAGGACA R: ACACTACACCATGCGGTCCT	[EMBL:AM275522]	Lachnospiraceae	137	2	65	85

ISSN: 1471-230X