Table 2 The primer sequences of each gene for RT-qPCR
From: The hsa_circ_0039857/miR-338-3p/RAB32 axis promotes the malignant progression of colorectal cancer
Gene | Primer sequence (5′–3′) | Accession number | annealing temperature |
|---|---|---|---|
circ_0039857 | F: CATCCAGAGGGAACCTGTGT | NM_020850 | 50 |
R: AAGTTGGCGTCCACAATCTC | |||
miR-338-3p | F: TGCGGTCCAGCATCAGTGATTTTGTT | NR_029897.1 | 45 |
R: CCAGTGCAGGGTCCGAGGT | |||
RT: GTCGTATCCAGTGCAGGGTCCG | |||
AGGTGCACTGGATACGACGGTCGTA | |||
RAB32 | F: CAGGTGGACCAATTCTGCAAA | NM_006834.5 | 48 |
R: GGCAGCTTCCTCTATGTTTATGT | |||
Caspase-1 | F: TTTCCGCAAGGTTCGATTTTCA | NM_001257119.3 | 45 |
R: GGCATCTGCGCTCTACCATC | |||
Bax | F: CCCGAGAGGTCTTTTTCCGAG | NM_001291430.2 | 50 |
R: CCAGCCCATGATGGTTCTGAT | |||
Bcl-2 | F: GGTGGGGTCATGTGTGTGG | NM_000633.3 | 45 |
R: CGGTTCAGGTACTCAGTCATCC | |||
GAPDH | F: GGAGCGAGATCCCTCCAAAAT | NM_001357943.2 | 45 |
R: GGCTGTTGTCATACTTCTCATGG | |||
U6 | F: CTCGCTTCGGCAGCACA | NR_004394.1 | 48 |
R: AACGCTTCACGAATTTGCGT | |||
RT: GTCGTATCCAGTGCAGGGTCCGA | |||
GGTGCACTGGATACGACTGGAACG |