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Fig. 1 | BMC Gastroenterology

Fig. 1

From: Exosomal microRNAs in the DLK1-DIO3 imprinted region derived from cancer-associated fibroblasts promote progression of hepatocellular carcinoma by targeting hedgehog interacting protein

Fig. 1

Characteristics of cancer-associated fibroblasts (CAFs) and CAFs-derived exosomes (CAFs-exo). A Representative morphology of CAFs and para-cancer fibroblasts (PAFs) isolated from hepatocellular carcinoma (HCC) patients. Scale bar: 200 μm. B Representative immunofluorescence (IF) images of α-SMA and Vimentin in CAFs and PAFs. Scale bar: 100 μm. C The protein expression levels of α-SMA and Vimentin in CAFs and PAFs were detected by western blot (WB) assay. D The protein expression levels of TSG101, CD81, and CD9 in CAFs-exo and PAFs-exo were detected by WB assay. E Size distribution of CAFs-exo and PAFs-exo was analyzed by nanoflow cytometry analysis. F Micrograph of CAFs-exo and PAFs-exo was visualized by transmission electron microscopy analysis. Scale bar: 200 nm. G Representative IF images of PLC/PRF/5 cells co-cultured with Dil-labelled CAFs. Scale bar: 50 μm

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