Fig. 6

MiRNA-374b-5p overexpression and inhibition affect the protein levels of IL-10 and p-STAT3 (Ser727). A The protein expressions of IL-10 and β-actin were tested by Western blotting in miRNA-374b-5p mimic- and inhibitor-transfected CD4⁺ T cells compared to NC-transfected cells, and the relative gray values were analyzed. B The protein levels of STAT3, p-STAT3 and β-actin were tested by Western blotting in miRNA-374b-5p mimic- and inhibitor-transfected CD4⁺ T cells compared to NC-transfected cells, and the relative gray values were analyzed. C The protein levels of JAK1 and β-actin were tested by Western blotting in miRNA-374b-5p mimic- and inhibitor-transfected CD4⁺ T cells, and the relative gray values were analyzed. (***P < 0.001, ****P < 0.0001; NS, not significant)