Table 2 Limit of detection for two methylated markers in ColoDefense2.0
From: A simplified multiplex methylated DNA testing for early detection of colorectal cancer in stool DNA
SDC2 | SEPT9 | ACTB | ||||||
|---|---|---|---|---|---|---|---|---|
CONC (pg/reaction) | PR (%) | Mean Ct | CONC (pg/reaction) | PR (%) | Mean Ct | CONC (pg/reaction) | PR rate (%) | Mean Ct |
0 | 0 | – | 0 | 0 | – | 20,000 | 100 | 25.6 ± 0.1 |
50 | 100 | 35.8 ± 0.4 | – | – | – | 5000 | 100 | 27.3 ± 0.1 |
25 | 100 | 37.6 ± 0.9 | – | – | – | 2500 | 100 | 28.9 ± 0.1 |
12.5 | 75 | 38.6 ± 1.2 | – | – | – | 1250 | 100 | 29.8 ± 0.2 |
– | – | – | 200 | 100 | 34.7 ± 0.2 | 20,000 | 100 | 25.7 ± 0.1 |
– | – | – | 100 | 100 | 36.2 ± 0.5 | 10,000 | 100 | 26.8 ± 0.2 |
– | – | – | 50 | 75 | 37.5 ± 0.8 | 5000 | 100 | 27.9 ± 0.2 |