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Fig. 2 | BMC Gastroenterology

Fig. 2

From: The upregulation of stromal antigen 3 expression suppresses the phenotypic hallmarks of hepatocellular carcinoma through the Smad3-CDK4/CDK6-cyclin D1 and CXCR4/RhoA pathways

Fig. 2

STAG3 overexpression inhibited cell proliferation, increased apoptosis and induced G1/S cell arrest in HCC. A The protein expression level of STAG3 in STAG3-OE HCC cells was detected by western blotting. B The mRNA expression level of STAG3 in STAG3-OE HCC cells was detected by RT–qPCR. C The effect of STAG3 overexpression on HCC cell proliferation was assessed by the CCK-8 assay. D The effect of STAG3 overexpression on HCC cell proliferation was assessed by a colony formation assay. The images represent colonies of cells belonging to the NC and STAG3-OE groups in six-well plates. E The apoptosis of STAG3-OE HCC cells was analyzed by flow cytometry. F The cell cycle of STAG3-OE HCC cells was analyzed by flow cytometry. *P < 0.05; **P < 0.01; ***P < 0.001

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