The gut microbiota composition in patients with right- and left-sided colorectal cancer and after curative colectomy, as analyzed by 16S rRNA gene amplicon sequencing

Table 4 Metabolic function prediction of the microbiota by PICRUSt2 of the SCC and control groups

Variable	P-value
Upregulation (SCC > C)
Ribitol-5-phosphate 2-dehydrogenase	0.0005
Lactocepin^c)	0.0061
Glutamyl aminopeptidase^a)	0.0067
Licheninase	0.0071
N-acylneuraminate-9-phosphate synthase^a)	0.0172
Maltose 6′-phosphate phosphatase^a)	0.0188
Sulfopropanediol 3-dehydrogenase	0.0192
Pyruvate dehydrogenase (acetyl-transferring)^a)	0.0320
Catechol 2,3-dioxygenase	0.0322
tRNA (uracil(54)-C(5))-methyltransferase	0.0340
Downregulation (C > SCC)
Lactaldehyde reductase	0.0051
Selenide, water dikinase	0.0069
Cobyrinate a,c-diamide synthase (glutamine-hydrolyzing)^d)	0.0081
Hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolyzing)	0.0081
Molybdate-transporting ATPase	0.0098
NAD(+) diphosphatase	0.0099
Threonine-phosphate decarboxylase	0.0108
Biotin carboxylase	0.0116
Adenosylcobyric acid synthase (glutamine-hydrolyzing)^d)	0.0116
Tagatose-bisphosphate aldolase^a)	0.0120

Using enzyme classification (EC) numbers, we tabulated the top 10 enzyme-encoding genes with highly significant differences between the SCC and control groups. Each metabolism-related enzyme was classified according to function within the range that could be discriminated as follows. ^a) sugar metabolism-related; ^c) short-chain fatty acid-related; and ^d) vitamin-related

ISSN: 1471-230X