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Fig. 3 | BMC Gastroenterology

Fig. 3

From: Nuclear respiratory factor 1 promotes the growth of liver hepatocellular carcinoma cells via E2F1 transcriptional activation

Fig. 3

Effect of NRF1 on cell proliferation. a HepG2 cells were transfected with siCtrl or siNRF1. NRF1 and β-actin were analysed by Western blot. b, c Plate colony formation assays and d MTT assays were used to measure the impact of NRF1 knockdown on cell clonality and proliferation. e The cell cycle distribution of HepG2 cells with stable NRF1 expression was analysed by flow cytometry. *P < 0.05, **P < 0.01 and ****P < 0.0001 compared with the control

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