Gene variants of the SLC2A5 gene encoding GLUT5, the major fructose transporter, do not contribute to clinical presentation of acquired fructose malabsorption

Table 1 Primers used for amplification and sequencing

Region^a	Sequence^b	fragment size (bp)	Different conditions from standard protocol^d
Promotor	M13-TCTCGCTCTGTCACCCA	456	60 °C + QS
	M13rev-GTCTTTGCCGTAGCCCA
Promotor	M13-TAACAGTAACAGAAACGCTCC	431	60 °C
	M13rev-CCTAGTGGCTCAAAGATGG
Promotor Exon 1 (UTR)^c	M13-GGTCTTGCTCTGTCACCT	324	58 °C + QS
	M13rev-CCCTTCAGCTTCTGCCA
Exon 2	M13-CCCACTTACTTAGCCAAACC	360
	M13rev-TTCCCTCTGCAACACCA
Exon 3	M13-TTGAGAAAGCCTGTAACCC	447	QS
	M13rev-CCCATCCCAAGAGACCT
Exon 4	M13-CAGGTTATTTCATTGGGTGTC	339
	M13rev-TGGTAAGGATTTCAGTTGTAGG
Exon 5	M13-CCACACTGAGCGTATTCC	448	58 °C + QS
	M13rev-GTTTCACAGCAGAGGTATAGG
Exon 6	M13-CCTTTGATCTGTTTCTCTTTCC	439	58 °C
	M13rev-AAAGTCCTGTCCTGTGGT
Exon 7	M13-AAAGCTGTGCCCTCCTG	402	58 °C + QS
	M13rev-CCTTCTCTGCCTCATCCTC
Exon 8	M13-TCTGCTGCCCTTCTTCC	574	QS
	M13rev-CATGACCACGTTCACGG
Exon 9	M13-CGTGCTGAAGCTGTTCC	474	QS
	M13rev-CAGAGTTTCTGTAGTAGCGG
Exon 10	M13-CTCAGGGTTGTGGGATTAGGA	640	QS
	M13rev-CAGACAAGCTAGGACGGGA
Exon 11	M13-CATCTGCCTCATAGCCTG	602	58 °C + QS
	M13rev-CTCATTATGTGCCACCCA
Exon 12, 13	M13-CCACATGCCCAAGAGTCCTG	730	58 °C + QS
	M13rev-AGCCCTTTGCACAGTTCCC

^aNumbering of exons is based on reference sequence NG_050918.1
^bSequencing tag M13 (gtaaaacgacggccagt) M13rev (ggaaacagctatgaccatg)
^cExon 1: untranslated region (UTR);
^dConditions refer to annealing temperature and addition of QS-solution (Qiagen, Hilden, Germany)