Skip to main content

Table 1 Primers used for amplification and sequencing

From: Gene variants of the SLC2A5 gene encoding GLUT5, the major fructose transporter, do not contribute to clinical presentation of acquired fructose malabsorption

Regiona Sequenceb fragment size (bp) Different conditions from standard protocold
Promotor M13-TCTCGCTCTGTCACCCA 456 60 °C + QS
  M13rev-GTCTTTGCCGTAGCCCA   
Promotor M13-TAACAGTAACAGAAACGCTCC 431 60 °C
  M13rev-CCTAGTGGCTCAAAGATGG   
Promotor
Exon 1 (UTR)c
M13-GGTCTTGCTCTGTCACCT 324 58 °C + QS
  M13rev-CCCTTCAGCTTCTGCCA   
Exon 2 M13-CCCACTTACTTAGCCAAACC 360  
  M13rev-TTCCCTCTGCAACACCA   
Exon 3 M13-TTGAGAAAGCCTGTAACCC 447 QS
  M13rev-CCCATCCCAAGAGACCT   
Exon 4 M13-CAGGTTATTTCATTGGGTGTC 339  
  M13rev-TGGTAAGGATTTCAGTTGTAGG   
Exon 5 M13-CCACACTGAGCGTATTCC 448 58 °C + QS
  M13rev-GTTTCACAGCAGAGGTATAGG   
Exon 6 M13-CCTTTGATCTGTTTCTCTTTCC 439 58 °C
  M13rev-AAAGTCCTGTCCTGTGGT   
Exon 7 M13-AAAGCTGTGCCCTCCTG 402 58 °C + QS
  M13rev-CCTTCTCTGCCTCATCCTC   
Exon 8 M13-TCTGCTGCCCTTCTTCC 574 QS
  M13rev-CATGACCACGTTCACGG   
Exon 9 M13-CGTGCTGAAGCTGTTCC 474 QS
  M13rev-CAGAGTTTCTGTAGTAGCGG   
Exon 10 M13-CTCAGGGTTGTGGGATTAGGA 640 QS
  M13rev-CAGACAAGCTAGGACGGGA   
Exon 11 M13-CATCTGCCTCATAGCCTG 602 58 °C + QS
  M13rev-CTCATTATGTGCCACCCA   
Exon 12, 13 M13-CCACATGCCCAAGAGTCCTG 730 58 °C + QS
  M13rev-AGCCCTTTGCACAGTTCCC   
  1. aNumbering of exons is based on reference sequence NG_050918.1
  2. bSequencing tag M13 (gtaaaacgacggccagt) M13rev (ggaaacagctatgaccatg)
  3. cExon 1: untranslated region (UTR);
  4. dConditions refer to annealing temperature and addition of QS-solution (Qiagen, Hilden, Germany)