Fig. 5

CTT positive but HRCM normal (negative) images in a representative FC patient (Case 33). A CTT positive in Case 33. B HRCM normal in Case 33. C HE staining showed constipation in Case 33. Left, intermuscular Auerbach nerve plexus atrophy was observed, with only a few atrophied nuclei. Right, nonspecific esterase staining (NSE) showed no neurons in the two Auerbach nerve plexuses